本文選題：YSTR + 單倍型�。� 參考：《遺傳學報》2002年02期

【摘要】：通過熒光標記引物結(jié)合ABI 377型全自動DNA測序儀檢測自動分型方法對中國壯族及漢族人群中各 10 0例無關(guān)男性個體的A10、C4、A7 1、A7 2等 4個Y染色體特異的基因座的等位基因及單倍型的分布進行了調(diào)查。結(jié)果發(fā)現(xiàn)A10、C4、A7 1、A7 2基因座分別有 7、6、6、6個等位基因 ,基因多樣性 (GD)分別為 0 7776 0 6 2 9(壯 漢 )、0 773 0 732、0 5 978 0 72 72、0 6 6 6 4 0 6 4 5 8。在 2 0 0個觀察樣本中共發(fā)現(xiàn) 114種單倍型 (haplotype) ,單倍型多樣性 (haplotypediversity,HD)分別為 0 9786 0 9772 (壯 漢 )。通過測序確認基因座核心重復序列及等位基因核心序列重復數(shù)。建立了這 4個基因座的復合擴增體系 ,分型準確清晰。還對這 4個基因座的男性特異性、遺傳穩(wěn)定性、靈敏度等法醫(yī)學有關(guān)指標進行了考察并且在實際案例中進行了應(yīng)用 ,結(jié)果證明 ,這 4個Y STRs基因座非常適應(yīng)于法醫(yī)檢驗 ,具有較高的實用價值
[Abstract]:Detection of four Y chromosomal specific loci in 100 unrelated male individuals in Chinese Zhuang nationality and Han nationality by fluorescent marker primer combined with ABI 377 automatic DNA sequencing apparatus for 4 Y chromosomal specific loci, such as A10C _ 4, A _ 4, A _ 7 _ 1, A _ 7 _ 2, etc.The distribution of site gene and haplotype was investigated.The results showed that there were 6 alleles and 6 alleles in A10C4, A7 1 and A7 2 loci, respectively, and the genetic diversity was 0 7776 629 (Chong Hou, 0 773 0 732, 0 5 978 0 72 72 2, 0 66 4 048 8), respectively.A total of 114 haplotype haplotypes were found in 200 observation samples. The haplotype diversity of haplotype was 0 9786 9772.The core repeats of loci and alleles were confirmed by sequencing.The multiplex amplification system of these four loci was established, and the typing was accurate and clear.The male specificity, genetic stability and sensitivity of the four loci were investigated and applied in practical cases.These four Y STRs loci are very suitable for forensic examination and have high practical value.
【作者單位】： 公安部物證鑒定中心 公安部物證鑒定中心 公安部物證鑒定中心 廣西刑事科學技術(shù)研究所
【基金】：公安部B類基金~~
【分類號】：D919

【共引文獻】

相關(guān)期刊論文 前10條

1 張艷霞;Y-STR——法醫(yī)學的新工具[J];山西醫(yī)科大學學報;2003年02期

2 王永在,張更謙,王艷,張艷霞,，

本文編號：1758840