本文選題：過敏性休克　切入點：類胰蛋白酶　出處：《吉林大學(xué)》2005年碩士論文

【摘要】：肥大細胞(Mast Cell,MC)與變態(tài)反應(yīng)性疾病有著密切的關(guān)系,在其發(fā)病過程中發(fā)揮重要的作用。過敏性休克是臨床常見的急癥之一,由于缺乏確切的實驗室診斷標準,是導(dǎo)致醫(yī)療糾紛的一個重要原因。因此,研究過敏性休克發(fā)生的機制,確立可靠、準確的診斷指標,提出簡單易行的診斷方法,在法醫(yī)病理診斷中尤為重要。本實驗應(yīng)用類胰蛋白酶、IL-1β免疫組化染色、甲苯胺藍特殊染色的方法觀察了27例過敏性休克致死和8例非過敏性休克致死病例的各臟器,通過研究,得到如下結(jié)論: 1.過敏性休克致死尸檢病例中各臟器肥大細胞(MC)數(shù)目增多,尤以肺組織、腸組織最明顯。 2.類胰蛋白酶可以作為MC特異性標記檢測組織中MC的數(shù)量。 3.尸檢病例腸及肺等組織中大量嗜酸性粒細胞的存在提示過敏性休克死亡。 4、TNF-α、IL-1β參與過敏性休克的病理過程。 5、過敏性休克時,腸粘膜Eotaxin染色陽性。 本實驗應(yīng)用免疫組化染色和特殊染色的方法,通過研究過敏性休克中MC的形態(tài)變化,證實在過敏性休克過程中類胰蛋白酶、TNF-α、IL-1β和Eotaxin發(fā)揮的作用,為其在法醫(yī)病理實踐中的應(yīng)用提供了切實的理論依據(jù)。
[Abstract]:Mast cell carcinoma (Mast) has a close relationship with allergic diseases and plays an important role in the pathogenesis of the disease. Anaphylactic shock is one of the common clinical emergencies, due to the lack of accurate laboratory diagnostic criteria. Therefore, to study the mechanism of anaphylactic shock, to establish reliable and accurate diagnostic indicators, and to put forward a simple and feasible diagnostic method. It is especially important in forensic pathology diagnosis. The organs of 27 cases of anaphylactic shock and 8 cases of non-anaphylactic shock were observed by using trypsinoid IL-1 尾 immunohistochemical staining and toluidine blue special staining. The following conclusions have been drawn:. 1. The number of mast cells in various organs was increased, especially in lung tissue and intestinal tissue. 2. Trypsin can be used as a specific marker to detect the number of MC in tissues. 3. The presence of a large number of eosinophils in intestinal and lung tissues suggests anaphylactic shock death. 4 TNF- 偽 -IL-1 尾 was involved in the pathological process of anaphylactic shock. 5. Eotaxin staining was positive in intestinal mucosa during anaphylactic shock. In this study, immunohistochemical staining and special staining were used to study the morphological changes of MC in anaphylactic shock. The results showed that the role of TNF- 偽 IL-1 尾 and Eotaxin in the process of anaphylactic shock. It provides a practical theoretical basis for its application in forensic pathology practice.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：D919

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