本文選題：法醫(yī)病理學(xué)　切入點(diǎn)：原發(fā)性腦干損傷　出處：《中國法醫(yī)學(xué)雜志》2011年05期 　論文類型：期刊論文

【摘要】：目的觀察原發(fā)性腦干損傷(PBSI)致死者腦干神經(jīng)元凋亡及caspase3的表達(dá)情況,探討其法醫(yī)學(xué)意義。方法選擇法醫(yī)學(xué)檢案中30例確定為原發(fā)性腦干損傷致死者腦干,按傷后不同存活時(shí)間(t≤5min、5mint≤15min、15mint≤1h、1ht≤24h)分為4組,以5例非腦干損傷死亡者腦干為對(duì)照組;分別運(yùn)用常規(guī)HE、免疫組化和TUNEL染色檢測(cè)中腦、橋腦及延腦重要核團(tuán)內(nèi)神經(jīng)元凋亡及caspase3的表達(dá)情況。結(jié)果原發(fā)性腦干損傷后15min～24h出現(xiàn)明顯神經(jīng)元細(xì)胞凋亡(23.2%～35.9%)和caspase3陽性表達(dá)(28%～54%),并呈現(xiàn)隨損傷時(shí)間進(jìn)行性增加的趨勢(shì),與對(duì)照組有顯著性差異(P0.01)。結(jié)論原發(fā)性腦干損傷早期即出現(xiàn)神經(jīng)元凋亡及caspase3表達(dá)增加,并隨損傷時(shí)間的延長呈進(jìn)行性增加,可據(jù)此輔助診斷原發(fā)性腦干損傷死亡。
[Abstract]:Objective to observe the apoptosis of brain stem neurons and the expression of caspase3 induced by primary brain stem injury (PBSI), and to explore its forensic significance. Methods Thirty patients with primary brain stem injury were selected. The rats were divided into 4 groups according to different survival time after injury: t 鈮，

本文編號(hào)：1638278