本文選題：DXYS基因座　切入點(diǎn)：單核苷酸變異　出處：《中華醫(yī)學(xué)遺傳學(xué)雜志》2005年05期 　論文類(lèi)型：期刊論文

【摘要】：目的調(diào)查DXYS267基因座遺傳多態(tài)性,研究該座位伴性遺傳單核苷酸的特征及其法醫(yī)學(xué)應(yīng)用價(jià)值。方法應(yīng)用聚合酶鏈反應(yīng)和聚丙烯酰胺凝膠電泳對(duì)DXYS267進(jìn)行群體調(diào)查,并作Hardy-Wein-berg平衡檢驗(yàn),計(jì)算法醫(yī)學(xué)群體遺傳學(xué)參數(shù)。依據(jù)該基因座伴性遺傳的單核苷酸變異,設(shè)計(jì)新的引物,選擇性擴(kuò)增Y染色體的DXYS267短串聯(lián)重復(fù)序列。結(jié)果在118名中國(guó)漢族無(wú)關(guān)個(gè)體中,共發(fā)現(xiàn)6個(gè)等位基因,基因型頻率分布符合Hardy-Weinberg平衡,基因座雜合度為0.6706,個(gè)體識(shí)別率為0.8433,非父排除率為0.5957。新引物可選擇性擴(kuò)增Y-短串聯(lián)重復(fù)(short tandemrepeat,STR)序列,在184名男性無(wú)關(guān)個(gè)體共發(fā)現(xiàn)4個(gè)等位基因,單倍型多樣性值為0.6372。結(jié)論DXYS267屬一類(lèi)X、Y染色體序列同源的STR序列位點(diǎn),是一個(gè)高度多態(tài)性的系統(tǒng)。同時(shí)利用該位點(diǎn)的伴性單核苷酸變異,選擇性擴(kuò)增Y染色體上的STR序列,用于親權(quán)鑒定和個(gè)人識(shí)別,尤其是混合斑男性檢材的分型和性別鑒定有實(shí)用價(jià)值。
[Abstract]:Objective to investigate the genetic polymorphism of DXYS267 locus, to study the characteristics of the locus associated genetic single nucleotide and its forensic application value. Methods Polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis (page) were used to investigate the population of DXYS267 and Hardy-Wein-berg equilibrium test was performed. The population genetic parameters of forensic medicine were calculated. According to the single nucleotide variation of locus ligand inheritance, a new primer was designed to selectively amplify the DXYS267 short tandem repeat sequence of Y chromosome. A total of 6 alleles were found, the genotype frequency distribution was consistent with Hardy-Weinberg equilibrium, the heterozygosity of locus was 0.6706, the individual recognition rate was 0.8433, and the non-paternal exclusion rate was 0.5957.The new primers could selectively amplify the Y- short tandem repeat short and repeat STRs. Four alleles were found in 184 male unrelated individuals. The haplotype diversity was 0.6372.Conclusion the STR loci of DXYS267 belong to a class of XY chromosomes homologous to each other. It is a highly polymorphic system. At the same time, the STR sequence on Y chromosome is selectively amplified by using the locus' associated single nucleotide variation for paternity identification and personal identification. In particular, the classification and sex identification of male samples with mixed spots are of practical value.
【作者單位】： 浙江省溫州醫(yī)學(xué)院法醫(yī)學(xué)教研室 華中科技大學(xué)同濟(jì)醫(yī)學(xué)院法醫(yī)系 北京市法庭技術(shù)鑒定研究所 武漢同濟(jì)醫(yī)院眼科教研室 華中科技大學(xué)同濟(jì)醫(yī)學(xué)院法醫(yī)系
【分類(lèi)號(hào)】：D919.1;

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳亞明;趙亞娣;;山西漢族人群Y-STR基因座DYS714的多態(tài)性研究[J];湖北民族學(xué)院學(xué)報(bào)(醫(yī)學(xué)版);2010年01期

2 王永在,張更謙,王艷,張艷霞,，

本文編號(hào)：1564470