發(fā)布時間：2018-03-03 11:21

  本文選題：鈍性心臟損傷　切入點：基質(zhì)金屬蛋白酶　出處：《山西醫(yī)科大學(xué)》2004年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 建立大鼠心臟鈍力性損傷模型；觀察損傷后正常區(qū),損傷區(qū)心肌的病理變化；MMP-1和MMP-2的表達強度出現(xiàn)的規(guī)律；試圖為心臟鈍力性損傷的法醫(yī)學(xué)鑒定提供客觀的形態(tài)學(xué)依據(jù)，并探討其可能的發(fā)生機制。 方法 健康成年Wistar大鼠50只隨機分為七組即0h,6h,12h，24h,3天，5天，7天，0h為對照組：開胸，暴露心臟，逐層關(guān)胸，，待各組成活至相應(yīng)時間時處死，取材。其余各組為：開胸，暴露心臟，打擊，逐層關(guān)胸，到實驗所需時間取心臟制作標本。HE染色，光鏡觀察；MMP-1及MMP-2免疫組織化學(xué)染色，計算機圖象分析，統(tǒng)計學(xué)分析。 結(jié)果 （1） 隨時間的延長，MMP-1和MMP-2的表達強度呈現(xiàn)規(guī)律變化：MMP-1在傷后12h開始明顯表達，逐漸增強，3d達高峰；MMP-2在傷后24h開始明顯表達，五天達高峰。（2）HE染色可見心肌組織淤血，出血，炎細胞浸潤，時間長的可有纖維化。（3）各損傷組圖象分析結(jié)果與對照組結(jié)果經(jīng)統(tǒng)計分析p＜0.05。 結(jié)論 （1）心臟損傷模型建立較成功；（2）對照組與實驗組HE染色結(jié)果與免疫組織化學(xué)結(jié)果有較好的一致性，各指標的分布規(guī)律說明MMP-1及MMP-2可作為檢測心肌損傷的指標加以研究。
[Abstract]:Objective to establish a rat model of cardiac blunt force injury, observe the pathological changes of myocardium in normal area and injured area after injury, and provide objective morphological basis for forensic identification of cardiac blunt force injury. The possible mechanism of its occurrence was also discussed. Methods Fifty healthy adult Wistar rats were randomly divided into seven groups: 0 h, 6 h, 12 h, 24 h, 3 days, 5 days, 7 days and 0 h as the control group: open chest, expose heart, close chest layer by layer, then kill each group after surviving to the corresponding time, the other groups were open chest and exposed heart. Beating, closing the chest layer by layer, taking the time to make heart specimen. HE staining, light microscope observation of MMP-1 and MMP-2 immunohistochemical staining, computer image analysis, statistical analysis. Results (1) the expression intensity of MMP-1 and MMP-2 showed a regular change with the extension of time, and the expression of MMP-1 began to appear at 12 hours after injury, gradually enhanced the expression of MMP-2 at the peak of 3 days after injury, and reached the peak at 24 hours after injury, and on the fifth day, congestion and bleeding of myocardial tissue were observed by HE staining. The results of image analysis of each injury group were compared with those of the control group (p < 0.05). Conclusion 1) the results of HE staining in the control group and the experimental group are in good agreement with the immunohistochemical results. The distribution of each index indicates that MMP-1 and MMP-2 can be used as indicators for the detection of myocardial injury.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2004
【分類號】：D919

【參考文獻】

相關(guān)期刊論文 前2條

1 杜文華,閔家新,朱佩芳,王正國;家兔實驗性心肌挫傷后早期左心功能與血流動力學(xué)變化規(guī)律的研究[J];中華創(chuàng)傷雜志;2002年09期

2 蔡建輝,易定華,劉維永,羅新林,梁繼河,何揚舉;撞擊參數(shù)對原發(fā)性心臟破裂傷的影響[J];中華創(chuàng)傷雜志;1995年02期

本文編號：1560836