本文關(guān)鍵詞：電致化學發(fā)光分子印跡傳感器的制備及其在毒品檢測中的應用　出處：《江南大學》2012年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 電致化學發(fā)光 分子印跡 聯(lián)吡啶釕 凝膠-溶膠 甲基苯丙胺 嗎啡 銥配合物

【摘要】：毒品泛濫已成全球的一大公害,每年約20萬人因濫用毒品致死,上千萬人喪失勞動力,嚴重破壞了社會發(fā)展與穩(wěn)定。如何在飛機、火車、海關(guān)等要地快速靈敏的識別出不法分子藏匿于行李、郵件的毒品,成為緝毒部門的一項重要任務。目前,對毒品的檢測方法有很多。但這些方法普遍存在儀器昂貴、樣品前處理過程復雜、檢測時間長等缺點,尤其是檢測限偏高,選擇性不好,對痕量毒品的檢測不夠靈敏。固相電致化學發(fā)光具有試劑用量少,靈敏度高等優(yōu)點,分子印跡技術(shù)具有良好的識別能力和富集能力。本文研究了將這兩種方法聯(lián)用的電致化學發(fā)光-分子印跡傳感器。具體內(nèi)容包括: 1.將電致化學發(fā)光檢測手段與分子印跡技術(shù)相結(jié)合,制備了一種高靈敏度、高選擇性的檢測甲基苯丙胺的電化學發(fā)光-分子印跡傳感器。首先在玻碳電極表面修飾發(fā)光配合物Ru(bpy)32+/Nafion/MWCNT得到電化學發(fā)光固相電極,然后在其基礎(chǔ)上制備對甲基苯丙胺具有特異性識別功能的sol-gel印跡膜。通過循環(huán)伏安,電化學發(fā)光,掃描電鏡等手段對傳感器性能進行了表征。該傳感器對目標分子具有高靈敏度和選擇性,線性范圍1.0×10~(-9) ~ 1.0×10~(-12) mol/L,檢測限可達1.0×10~(-15) mol/L。能夠檢測出密閉容器中揮發(fā)出來的痕量甲基苯丙胺。 2.制備了一種測定嗎啡的新型ECL-MIP傳感器,首先基于MWCNT/Titania /Nafion復合膜將聯(lián)吡啶釕固定在電極表面,得到固相發(fā)光電極,然后再修飾一層選擇性印跡膜,該傳感器可對溶液中的嗎啡分子進行特異性識別和富集,具有很高的檢測靈敏度和優(yōu)異的選擇性,線性范圍1.0×10~(-6) ~ 1.0×10~(-9) mol/L,檢測限可達2.0×10~(-10) mol/L。并且該傳感器具有良好的穩(wěn)定性及再生能力,有望成為一種靈敏快速、選擇性高、再生性好的嗎啡分析新方法。 3.采用一種新型金屬銥配合物(ppy)_2Ir(N-phBA)(其中ppy = 2-苯基吡啶,N-phBA = N-苯基苯酰胺)作為發(fā)光試劑,以三丙胺為共反應物,探討了其在乙腈溶液中的電化學及電致化學發(fā)光行為。制備了多壁碳納米管/銥(III)修飾電極,對其電致發(fā)光性能及穩(wěn)定性進行考察。結(jié)果表明,(ppy)_2Ir(N-phBA)是一種優(yōu)良的電致化學發(fā)光試劑,發(fā)光強度較大且背景發(fā)光低;多壁碳納米管/銥(III)修飾電極制作簡單,容易保存,穩(wěn)定性好,可望用于含氮有機物的在線快速測定,在電致化學發(fā)光領(lǐng)域具有潛在的分析應用價值。
[Abstract]:Drug abuse has become a major public hazard in the world, about 200,000 people die of drug abuse every year, tens of millions of people have lost their labor force, which has seriously damaged social development and stability. How to fly and train. Customs and other important places to quickly and sensitively identify illegal elements hiding in luggage, mail drugs, has become an important drug department task. There are many methods for the detection of drugs, but these methods have many disadvantages, such as expensive instruments, complex sample pretreatment process, long detection time, especially high detection limit and poor selectivity. The detection of trace drugs is not sensitive enough. Solid-phase electrochemiluminescence has the advantages of less reagent dosage and high sensitivity. Molecular imprinting technology has good recognition ability and enrichment ability. In this paper, electrochemiluminescence and molecular imprinting sensors combined with these two methods are studied. The main contents are as follows: 1. A kind of high sensitivity was prepared by combining electrochemiluminescence with molecular imprinting. A highly selective electrochemiluminescence sensor for the detection of methamphetamine-molecularly imprinted sensor. The first step is to modify the luminescent complex Runbpyy on the surface of glassy carbon electrode. 32 / Nafion / MWCNT electroluminescent solid state electrode was obtained. Then the sol-gel imprinted membrane with specific recognition function for methamphetamine was prepared on the basis of the membrane. The electrochemiluminescence (ECL) was obtained by cyclic voltammetry. The performance of the sensor was characterized by scanning electron microscopy. The sensor has high sensitivity and selectivity to the target molecule. The linear range is 1.0 脳 10 ~ (-9) ~ 1.0 脳 10 ~ (10 ~ (-12)) mol/L. The detection limit is up to 1.0 脳 10 ~ (-1) -15) mol 路L ~ (-1) 路L ~ (-1). Trace methamphetamine volatilized in a closed vessel can be detected. 2. A novel ECL-MIP sensor for the determination of morphine was prepared. Firstly, bipyridine ruthenium was immobilized on the electrode surface based on MWCNT/Titania / Nafion composite membrane. Solid-phase luminescent electrode was obtained and then modified with a selective imprinted membrane. The sensor can specifically identify and enrich morphine molecules in the solution with high detection sensitivity and excellent selectivity. The linear range is 1.0 脳 10 ~ (-6) ~ 1.0 脳 10 ~ (10 ~ (-9)) mol/L. The detection limit is up to 2.0 脳 10 ~ (-10) mol 路L ~ (-1) mol 路L ~ (-1). The sensor has good stability and regeneration ability, so it is expected to be a sensitive, rapid and highly selective sensor. A new method for morphine analysis with good reproducibility. 3. A new metal iridium complex (ppy = 2-phenylpyridine) was used. N-phBA = N-phenylbenzamide was used as luminescent reagent and tripropylamine was used as co-reactant. The electrochemistry and electrochemiluminescence behavior of multiwalled carbon nanotube / iridium iridium II modified electrode in acetonitrile solution were investigated. The electroluminescent properties and stability of the electrode were investigated. It is a kind of excellent electrochemiluminescence reagent with high luminescence intensity and low background luminescence. The multiwalled carbon nanotube / iridium iridium iridium modified electrode is simple, easy to preserve and stable. It is expected to be used in the on-line rapid determination of nitrogen-containing organic compounds and has potential application value in electrochemiluminescence.
【學位授予單位】：江南大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：D919.1;TP212

【引證文獻】

相關(guān)碩士學位論文 前1條

1 孫佳妮;環(huán)丙沙星分子印跡聚合物的計算模擬與制備研究[D];吉林農(nóng)業(yè)大學;2013年

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本文編號：1409713