生物冶金是指在相關(guān)微生物存在時(shí)由于微生物的催化氧化作用將礦物中有價(jià)金屬以離子形式溶解到浸出液中加以回收或?qū)⒌V物中有害元素溶解并除去的方法。因其處理成本相對(duì)較低、環(huán)境污染小、反應(yīng)溫和等優(yōu)點(diǎn)、在難處理金礦預(yù)處理過(guò)程中得到廣泛關(guān)注。本研究將嗜鐵鉤端螺旋菌作為預(yù)處理用微生物,系統(tǒng)研究其與硫砷金精礦的生物氧化過(guò)程。首先研究嗜鐵鉤端螺旋菌ATCC 49881在9K培養(yǎng)基中的生長(zhǎng)情況,確定最優(yōu)生物氧化活性的生長(zhǎng)條件,得出以下結(jié)論：1.嗜鐵鉤端螺旋菌ATCC 49881在9K培養(yǎng)基中的生長(zhǎng)情況表明：不同初始pH值下,嗜鐵鉤端螺旋菌ATCC 49881指數(shù)期發(fā)生在12h至50h,在培養(yǎng)時(shí)間為97h時(shí),嗜鐵鉤端螺旋菌菌體密度達(dá)到最大(約8.2 x 106細(xì)胞/ml、5.6 x 106細(xì)胞/ml、6.4 x 106細(xì)胞/ml為初始pH值分別為1.6、pH值1.8和pH為2.0)。2.通過(guò)使用Lineweaver burk進(jìn)行擬合得到嗜鐵鉤端螺旋菌的莫諾方程。由莫諾方程可知,當(dāng)初始pH值為1.6、pH值1.8和pH值2.0時(shí)、菌體的最大比生長(zhǎng)速率(μmax)分別為0.076 h-1、0.178 h-1、0.0...

【文章頁(yè)數(shù)】：93 頁(yè)

【學(xué)位級(jí)別】：碩士

【文章目錄】：
摘要
ABSTRACT
CHAPTER Ⅰ INTRODUCTION
    1.1 Background
        1.1.1 Bioleaching
        1.1.2 Development of bioleaching
        1.1.3 Development of bioleaching in China
    1.2 Brief description of gold ore
        1.2.1 Refractory of gold ore
        1.2.2 Factor affecting leaching performance
    1.3 Bioleaching and biooxidation mechanisms
    1.4 Major species of microorganisms in bioleaching
        1.4.1 Characteristic of Leptospirillum ferriphium
    1.5 Microbial growth and its dynamic
    1.6 Basic concept on microbial kinetics of iron oxidation
    1.7 Basic concept on microbial kinetics of arsenopyrite oxidation
    1.8 Aims and Objective of Research
        1.8.1 Aims and objectives
        1.8.2 Research scope
CHAPTER Ⅱ MATERIALS AND METHODS
    2.1 Materials
        2.1.1 Arsenopyrite-bearing gold concentrate characterization
    2.2 Equipment and chemical reagents
        2.2.1 Equipment
        2.2.2 Chemical reagents
    2.3 Methodology of research
        2.3.1 Experimental procedures
        2.3.2 Experimental apparatus
        2.3.3 Microbial culture
    2.4 Analytical Technique
        2.4.1 Determination of bacterial cell density by direct microscopic counting
        2.4.2 pH and oxidation reduction potential
        2.4.3 Spectrophotometric of iron assay
        2.4.4 Total dissolved arsenic assay
    2.5 Sample collections and data handling
CHAPTER Ⅲ MICROBIAL ACTIVATION AND MICROBIAL GROWTH
    3.1 Leptospirillum ferriphilum activation
    3.2 Leptospirillum ferriphilum growth
    3.3 The kinetics study
        3.3.1 Cell growth kinetics
        3.3.2 Kinetics of ferrous iron biooxidation
    3.4 Summary
CHAPTER Ⅳ BIOOXIDATION EXPERIMENTS OFARSENOPYRITE-BEARING GOLD CONCENTRATE
    4.1 Preliminary bioleaching test
    4.2 Biooxidation optimization
        4.2.1 Pretreatment of arsenopyrite-bearing gold concentrate with variation of chemical compound
        4.2.2 Pretreatment of arsenopyrite-bearing gold concentrate with variation of ferrous iron(Fe²⁺)
        4.2.3 Pretreatment of arsenopyrite-bearing gold concentrate with variation of ferric iron(Fe³⁺)
    4.3 The kinetics study of the biooxidation of arsenopyrite-bearing gold concentrate
    4.4 Summary
CHAPTER Ⅴ CLOSURE
    5.1 Conclusion
    5.2 Suggestions
REFERENCES
APPENDICES
致謝
攻讀碩士學(xué)位期間發(fā)表的學(xué)術(shù)論文目錄



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