堿性螺旋-環(huán)-螺旋（bHLH）蛋白組成了一個(gè)存在于真核生物中的轉(zhuǎn)錄因子超家族,在微小如酵母、大到如人類的生物中都有發(fā)現(xiàn)。它們?cè)谡{(diào)控真核生物的神經(jīng)發(fā)育、性別決定、肌肉生成、細(xì)胞命運(yùn)決定以及響應(yīng)環(huán)境信號(hào)等發(fā)育過程中發(fā)揮重要作用。bHLH這一名稱來自于高度保守的結(jié)構(gòu)基序,即包含約60個(gè)氨基酸、由一個(gè)堿性（basic）區(qū)域和兩個(gè)被可變長(zhǎng)度環(huán)分開的螺旋區(qū)域（HLH）組成的結(jié)構(gòu)域。目前,根據(jù)bHLH蛋白與DNA結(jié)合的特性將其分為6個(gè)大組;此外,根據(jù)它們?cè)谡{(diào)控不同發(fā)育與生理過程中的作用將它們分為45個(gè)家族。地中海果蠅（Ceratitis capitata）是廣泛分布于世界各地的一種果蠅。它們以水果為食并在其中繁殖,因而對(duì)水果造成很大的物理及經(jīng)濟(jì)損害。為了控制地中海果蠅的危害,了解其生長(zhǎng)發(fā)育機(jī)理具有重要意義。地中海果蠅的基因組測(cè)序計(jì)劃在2016年9月即已完成,而其基因組中編碼了多少個(gè)bHLH基因還未見報(bào)道。因此,我們開展了Blast搜索與系統(tǒng)發(fā)生分析來建立地中海果蠅bHLH（CcbHLH）基因的清單。無剛毛-盾片復(fù)合體（achaete-scute complex,ASC）基因編碼在神經(jīng)發(fā)育中發(fā)揮重要作... 

【文章來源】：江蘇大學(xué)江蘇省

【文章頁數(shù)】：67 頁

【學(xué)位級(jí)別】：碩士

【文章目錄】：
ACKNOWLEDGEMENT
ABSTRACT
摘要
List of Abbreviations
Chapter1 Introduction
    1.1 bHLH transcription factors
        1.1.1 Animal bHLH transcription factors
        1.1.2 Insect bHLH family members
    1.2 Achaete-scute complex genes
        1.2.1 Functions of achaete-scute complex genes
        1.2.2 Classification of achaete-scute complex genes
    1.3 The Mediterranean fruit fly
        1.3.1 Morphology and habitat
        1.3.2 Reproduction and development
    1.4 Insects and food production
        1.4.1 Pests control in agriculture
        1.4.2 Insects as food
    1.5 Objectives and significance of this study
        1.5.1 Objectives
        1.5.2 Significance
    1.6 Research programs and technological schemes
        1.6.1 Research programs
        1.6.2 Technological schemes
Chapter2 Identification and phylogenetic analyses of Mediterranean fruit fly bHLH genes
    2.1 Materials and methods
        2.1.1 The45 representative bHLH motifs
        2.1.2 Blastp searches
        2.1.3 Multiple sequence alignment
        2.1.4 Phylogenetic analysis
        2.1.5 tBlastn searches
        2.1.6 Intron location
    2.2 Results and discussion
        2.2.1 Identification of CcbHLH family members
        2.2.2 Identification of orthologous families
        2.2.3 Identification of CcbHLH protein sequences
        2.2.4 Genomic coding regions of CcBHLH motifs
    2.3 Summary
Chapter3 Identification and phylogenetic analyses of insect achaete-scute complex genes
    3.1 Materials and methods
        3.1.1 Insect and other animal species
        3.1.2 Search and identification of ASC family members
        3.1.3 Phylogenetic analysis
        3.1.4 Display of conservative amino acids
        3.1.5 Location of ASC family genes
    3.2 Results and discussion
        3.2.1 New ASC gene family
        3.2.2 bHLH gene duplication in ASCa family
        3.2.3 Evolution of ASC genes in insects
    3.3 Summary
Chapter4 Conclusions and perspectives
    4.1 Conclusions
    4.2 Perspectives
References
Publications at Jiangsu University
Appendix



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