近年來,由化學(xué)殘留物導(dǎo)致的食品安全問題引起了全世界的極大擔(dān)憂。農(nóng)藥是農(nóng)業(yè)生產(chǎn)的重要資料,但是,會導(dǎo)致農(nóng)產(chǎn)品內(nèi)外污染農(nóng)藥殘留物。因此,有必要開展檢測農(nóng)藥痕量殘留物的技術(shù)研究。傳統(tǒng)的理化分析方法耗時、精細(xì)并且需要繁瑣的樣品制備,開展農(nóng)藥殘留的快速檢測技術(shù)研究對確保農(nóng)產(chǎn)品安全具有重要意義。因此,本論文主要側(cè)重于表面增強拉曼光譜（SERS）用于檢測最受歡迎和廣泛的消費水果的桃果實中不同的有機磷農(nóng)藥研究。在論文的第一部分,使用簡單快速的表面增強拉曼光譜（SERS）方法與銀膠體基底一起檢測標(biāo)準(zhǔn)溶液和桃提取物中的氧化樂果。研究結(jié)果表明,使用SERS方法可以精確識別農(nóng)藥的特征波數(shù),并計算出的標(biāo)準(zhǔn)溶液和桃提取物中氧化樂果的檢出限分別為0.001 mg/L和0.01 mg/kg。在論文的第二部分,對不同核心尺寸的雙金屬納米粒子進(jìn)行了優(yōu)化,以獲得最高的SERS效應(yīng),并快速檢測桃果實中的有機磷農(nóng)藥（三唑磷和甲基對硫磷）。首先,將所選Au@Ag NPs的拉曼增強效應(yīng)與純的Ag NPs和Au NsP進(jìn)行比較,研究表明Au NPs的尺寸顯著影響拉曼信號增強。發(fā)現(xiàn)具有（26nm Au核心尺寸和6nm Ag殼厚度）的A... 

【文章來源】：華南理工大學(xué)廣東省 211工程院校 985工程院校 教育部直屬院校

【文章頁數(shù)】：124 頁

【學(xué)位級別】：博士

【文章目錄】：
摘要
ABSTRACT
LIST OF ABBREVIATIONS
CHAPTER 1 INTRODUCTION AND LITERATURE REVIEW
    1.1 Introduction
    1.2 Peach Fruit
    1.3 Effect of Pesticides
        1.3.1 Organophosphorus Pesticides
        1.3.2 Detection Methods for Pesticides
        1.3.3 Raman Spectroscopy
    1.4 DEVELOPMENT OF SERS
        1.4.1.Roles of Substrates in SERS
            1.4.1.1.Colloidal substrates
            1.4.1.2.Solid substrates
    1.5 Applications of SERS on Food Quality and Safety
        1.5.1.Fruit and Vegetables
        1.5.2.Meat
        1.5.3.Dairy products
        1.5.4.Fishery products
    1.6 Project and thesis
        1.6.1 Need for the Projects
        1.6.2 Objectives
        1.6.3 Thesis structure
CHAPTER 2 EXPERIMENTAL WORK
    2.1 MATERIAL AND METHODS
        2.1.1 Chemicals and reagents
    2.2 Synthesis and characterization of substrates
        2.2.1 Silver colloid
        2.2.2 Gold colloid
        2.2.3 Core-shell nanoparticles
        2.2.4 Characterization of substrates
    2.3 Preparation of Standard solution
    2.4 Extraction of pesticides from peach
    2.5 SERS spectral collection
    2.6 Data analysis
        2.6.1 Spectral data pre-processing
        2.6.2 Data analysis
CHAPTER 3 DETECTION OF OMETHOATE IN PEACH WITH SERS
    3.1 INTRODUCTION
    3.2 MATERIAL AND METHODS
        3.2.1 Chemicals and reagents
        3.2.2 Synthesis and characterization of silver colloid
        3.2.3 Standard solution
        3.2.4 Extraction of omethoate from peach
        3.2.5 SERS spectral collection approach
        3.2.6 Data analysis
    3.3 RESULTS AND DISCUSSION
        3.3.1 Spectral features of omethoate
        3.3.2 SERS analysis of standard solutions
        3.3.3 SERS analysis of omethoate in peach extract
    3.4 CONCLUSIONS
CHAPTER 4 ENHANCEMENT EFFECT OF SERS FOR DETECTION OF TRIAZOPHOS AND METHYL-PARATHION RESIDUES IN PEACH BASED ON CORE-SHELL BIMETALLIC AU@AGNPS
    4.1 INTRODUCTION
    4.2 MATERIAL AND METHODS
        4.2.1 Chemicals and reagents
        4.2.2 Synthesis and characterization of substrates
        4.2.3 Characterization of substrates
        4.2.4 Standard solution
        4.2.5 Extraction of pesticide from peach
        4.2.6 SERS spectral collection
        4.2.7 Data analysis
    4.3 RESULTS AND DISCUSSION
        4.3.1 Spectral features of triazophos and parathion-methyl
        4.3.2 Characterization of substrates
        4.3.3 Selection of different size Au@Ag nanoparticles for triazophos and parathion-methyl
        4.3.4 Raman enhancement mechanism of Au@Ag NPs
        4.3.5 Analysis of triazophos and parathion-methyl standard solutions
        4.3.6 Analysis of triazophos and parathion-methyl in peach extract
        4.3.7 Recovery of pesticide concentration in peach extract
        4.3.8 Interference evaluation of other pesticides
        4.3.9 Comparison of the current Au@Ag NPS-SERS method with other reported methods
    4.4 CONCLUSIONS
CHAPTER 5 SIMULTANEOUS DETECTION OF MULTI-CLASS INSECTICIDE RESIDUES IN PEACH WITH SERS TECHNIQUE BASED ON CORE-SHELL BIMETALLIC AU@AG NPS
    5.1 INTRODUCTION
    5.2 MATERIAL AND METHODS
        5.2.1 Chemicals and reagents
        5.2.2 Synthesis and characterization of substrates
        5.2.3 Characterization of substrates
        5.2.4 Standard solution
        5.2.5 Extraction of pesticide from peach
        5.2.6 SERS measurement
        5.2.7 Data analysis
    5.3 RESULTS AND DISCUSSION
        5.3.1 Spectral features of thiacloprid,profenofos and oxamyl
        5.3.2 Characterization of substrates
        5.3.3 SERS analysis of single insecticide standard solutions
        5.3.4 SERS analysis of single insecticide in peach extract
        5.3.5 Recovery of pesticide concentration in peach extract
        5.3.6 Simultaneous detection of multi-class insecticides in peach extracts
    5.4 CONCLUSIONS
CHAPTER 6 EFFECTS OF IONS ON CORE-SHELL BIMETALLIC AU@AG NPS FOR DETECTION OF PHOSALONE IN PEACH BY SERS
    6.1 INTRODUCTION
    6.2 MATERIALS AND METHODS
        6.2.1 Chemicals and reagents
        6.2.2 Synthesis and characterization of substrates
        6.2.3 Characterization of substrates
        6.2.4 Detection of phosalone standard solution
        6.2.5 Extraction of phosalone from peach
        6.2.6 SERS spectral collection
        6.2.7 Data analysis
    6.3 RESULTS AND DISCUSSION
        6.3.1 Spectral features of phosalone
        6.3.2 Characterization of substrates
        6.3.3 Effects of concentrations of aggregating agents on SERS intensity
        6.3.4 Effects of pH on SERS intensity
        6.3.5 Effects of different aggregating agents on SERS intensity
        6.3.6 Detecting phosalone in standard solutions
        6.3.7 Detecting phosalone in peach extract
    6.4 CONCLUSIONS
CHAPTER 7 CONCLUSIONS AND RECOMMENDATIONS
    7.1 CONCLUSION
    7.2 ACHIEVEMENTS AND INNOVATIONS
    7.3 RECOMMENDATION FOR FUTURE WORK
REFERENCES
攻讀博士學(xué)位期間取得的研究成果
ACKNOWLEDGMENTS
附件


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期刊論文
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