高羊茅（Festuca arundinacea Schreb.）是一種冷季型草坪草,對于低溫的耐受性相對較強,但是高羊茅的抗熱性和抗旱性在草坪草中屬于較差類型。華中地區(qū)高羊茅的越夏困難,如何提高高羊茅對高溫和干旱的抗性具有重要的意義。褪黑素（MT）和油菜素內(nèi)酯（BR）作為兩種重要的生長調(diào)節(jié)物質(zhì),都可以促進植物的生長,增強植物的抗逆性。本研究中,利用MT和2,4-油菜素內(nèi)酯（EBL）處理高羊茅材料,探討MT和EBL對高羊茅生長狀態(tài)、生理指標以及基因表達的影響。研究結(jié)果如下:1.高溫處理實驗中,8天苗齡的高羊茅材料用20 μM MT或0.1 μM EBL預(yù)處理2天,然后在38 ℃條件下進行處理6或12小時。結(jié)果表明,在高溫處理以后,MT和EBL預(yù)處理的高羊茅材料展示出較低的電導(dǎo)率、MDA含量、活性氧含量,包括超氧陰離子（O2-）和過氧化氫（H2O2）,但是較高的葉綠素含量、總蛋白質(zhì)含量和抗氧化酶活性（包括超氧化物歧化酶、過氧化氫酶和過氧化物酶）。高溫條件下MT和EBL預(yù)處理促進了高羊茅的生長,提高了植株的鮮重和植株高度。通過RNA seq分析發(fā)現(xiàn),4311和8395個unigene在高溫處... 

【文章來源】：中國科學院大學(中國科學院武漢植物園)湖北省

【文章頁數(shù)】：137 頁

【學位級別】：博士

【文章目錄】：
摘要
Abstract
1. Background
    1.1 Heat stress
        1.1.1 Effects of heat stress on the plants
        1.1.2 The roles of ROS during plant heat stress responses
        1.1.3 Adaptation mechanisms of plants to hightemperature
        1.1.4 Effects of exogenous compounds on plant heat stress responses
    1.2 Drought stress
    1.3 Melatonin
        1.3.1 Functions of melatonin
        1.3.2 Biosynthesis of melatonin
        1.3.3 The roles of melatonin
    1.4 2,4-Epibrassinolide
        1.4.1 Functions of brassinosteroids
        1.4.2 The roles of 2,4-epibrassinolide/brassinosteroids
    1.5 Tall fescue
2. Chapter OneTranscriptomis profiling of tall fescue in response to heat stress andimproved thermotolerance by melatonin and 2,4 epibrasnolide
    2.1 Introduction
        2.1.1 Objectives, purposes and content research
    2.2 Materials and Methods
        2.2.1 Plant material and growth conditions
        2.2.2 Chemicals, treatments and experimental design
        2.2.3 Measurement of fresh weight and plant height of tall fescue
        2.2.4 Quantification of reactive oxygen species,antioxidantenzymes and protein content
        2.2.5 Measurement of malondialdehyde and electrolyte leakage
        2.2.6 Measurement of chlorophyll
        2.2.7 RNA sequencing analysis
        2.2.8 Gene ontology (GO)term and pathway enrichment and clusteranalyses
        2.2.9 Quantitative real-time-polymerasc chain reaction (qRT-PCR)
        2.2.10 Statistical analysis
    2.3 Result
        2.3.1 Effects of MT and EBL on the plant growth under heatstress
        2.3.2 Effects of MT and EBL on electrolyte leakage, chlorophyll andtotal protein content under heat tress
        2.3.3 Effects of MT and EBL on ROS and cell membrane stabilityunder heat stress
        2.3.4 Effects of MT and EBL priming on the activities of antioxidantenzymes under heat stress
        2.3.5 General transcriptomic responses of tall fescue to heat stress
        2.3.6 GO term and pathway enrichment analyses
        2.3.7 Cluster analysis and functions of heat stress responsivegenes
        2.3.8 Changes of hormone pathway related genes
        2.3.9 Effects of MT and EBL on the expression of heat stress inducedgenes
    2.4 Discussion
3 Chapter TwoMelatonin ameliorates drought stress-induced damages of tall fescue
    3.1 Introduction
        3.1.1 Objectives
    3.2 Materials and Methods
        3.2.1 Plant materials and growth conditions
        3.2.2 Experimental design and drought stress treatment
        3.2.3 Measurement of shoot fresh weight and shoot height of tallfescue Seedlings
        3.2.4 Measurement of water loss and leaf water content
        3.2.5 Quantification of reactive oxygen species and antioxidantenzymes
        3.2.6 Measurement of malondialdehide and electrolyte leakage
        3.2.7 Measurement of chlorophyll
        3.2.8 Statistical analysis
    3.3 Result
        3.3.1 Effects of exogenous melatonin an growth conditions of tallfescue seedling
        3.3.2 Effects of exogenous melatonin on electrolyte leakage and totalchlorophyll content
        3.3.3 Effects of exogenous melatonin on shoot water loss and shootwater content
        3.3.4 Effects of exogenous melatonin on ROS and cell membranestability
        3.3.5 Effects of exogenous melatonin on the antioxidant enzymeactivities
    3.4 Discussion
4. Conclusion
5. References
Appendices
Student profile
Acknowledgement


【參考文獻】：
期刊論文
[1]Interactive effects of drought stress and chitosan application on physiological characteristics and essential oil yield of Thymus daenensis Celak[J]. Zohreh Emami Bistgani,Seyed Ataollah Siadat,Abdolmehdi Bakhshandeh,Abdollah Ghasemi Pirbalouti,Masoud Hashemi.  The Crop Journal. 2017(05)
[2]外源褪黑素對高溫脅迫條件下黃瓜幼苗氮代謝的影響[J]. 趙娜,孫艷,王德玉,鄭俊鶱.  植物生理學報. 2012(06)



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