C₄植物的光合作用效率高于C₃途徑的植物接近50%。為了將C₄途徑引入C₃植物中,需要對調控光合作用的關鍵基因的分子作用機理進行研究,特別是了解這些基因葉肉細胞(Mc)和維管束鞘細胞(BSc)中差異表達。因此,在本實驗中的研究目的是:⑴從水稻和玉米中獲得均一Mc和BSc,以便通過利用LCM技術進行細胞特定RNA測序;⑵分析水稻作為C₃植物的特定Mc和BSc基因的差異表達;⑶分析玉米作為C₄植物的特定Mc和BSc基因的差異表達。水稻中已經鑒定了包括Mc和BSc總共35956個基因,其中BSc特有2068個,Mc特有5842個。參與光合作用的基因有:在Mc中特異性表達的OsSET13與OsSET26(Rubisco LS甲基轉移酶底物結合域)、CAB2R/OsLhcb1.2與RCABP89/OsLhcb2(葉綠素A-B結合蛋白)、PsaH與PsbX(光系統(tǒng)組分)、未知功能CHP01589、K09122、OsPM8ATP基因,但在BSc中這些基因也往往存在微量表...

【文章頁數】：86 頁

【學位級別】：博士

【文章目錄】：
摘要
abstract
Chapter1 INTRODUCTION
    1.1 Photosynthetic pathways
        1.1.1 C₃ pathway
        1.1.2 C₄ pathway
    1.2 Evolution of C₄ photosynthetic pathway
        1.2.1 Phylogenetic distribution
        1.2.2 Kranz anatomy
        1.2.3 Molecular regulation of C₄ pathways
    1.3 The C₄ Rice Project
        1.3.1 Rice
        1.3.2 The C₄ Rice Project – Overview
        1.3.3 Previous studies
    1.4 Laser capture microdissection technique
        1.4.1 LCM systems
        1.4.2 LCM applications on plants
    1.5 Aims
Chapter2 MATERIALS AND METHODS
    2.1 Plant materials
        2.1.1 Oryza sativa
        2.1.2 Zea mays
    2.2 LCM sample preparation
        2.2.1 Leaf sample collection and fixation
        2.2.2.Microwave processing
        2.2.3 Microtome sectioning
        2.2.4 LCM
    2.3 RNA Extraction and qualification
        2.3.1 RNA extraction
        2.3.2 RNA integrity testing
    2.4 RNA amplification and RNA-seq
        2.4.1 RNA amplification
        2.4.2 RNA-seq
Chapter3 Mc- and BSc-specific RNA of rice and maize
    3.1 Introduction
    3.2 Results and discussion
        3.2.1 Leaf collection
        3.2.2 Laser capture microdissection
        3.2.3 RNA Extraction and RNA Integrity Testing
    3.3 Discussion
Chapter4 Differential expression of rice Mc and BSc transcriptomes
    4.1 Introduction
    4.2 Results
        4.2.1 RNA-seq and alignment against the rice reference genome
        4.2.2 Differential expression of genes between rice BSc and Mc
    4.3 Discussion
        4.3.1 RNA-seq and alignment against the reference genome
        4.3.2. Differential expression of genes between BSc and Mc
Chapter5 Differential expression of maize Mc and BSc transcriptomes
    5.1 Introduction
    5.2 Results
        5.2.1 RNA-seq and alignment against the reference genome
        5.2.2 Differential expression of genes between BSc and Mc
    5.3 Discussion
        5.3.1 RNA-seq and alignment against the reference genome
        5.3.2 Differential expression of genes between BSc and Mc
CONCLUSION
References
致謝Acknowledgement
作者簡歷RESUME



本文編號：3992014