了解作物對鹽脅迫的生理生化反應(yīng)有助于提高作物生長性能,增加作物產(chǎn)量。在本研究中,我們研究了不同類型鹽脅迫下野大豆和栽培大豆幼苗的生長、光合作用參數(shù)、離子濃度以及參與氮同化相關(guān)酶活性的變化。研究結(jié)果顯示,兩種類型鹽脅迫均會對野大豆和栽培大豆的生長和代謝產(chǎn)生不利影響。生長和光合參數(shù)隨鹽濃度的增加而顯著降低。此外,根部鈉(Na⁺)和氯(Cl^-)濃度顯著增加。然而,在中性鹽和堿性鹽脅迫下,野大豆光合性能以及離子調(diào)控能力顯著高于栽培大豆.在野大豆和栽培大豆幼苗葉片中,硝酸還原酶(NR)活性和谷氨酰胺合成酶/谷氨酰胺氧谷氨酸氨基轉(zhuǎn)移酶(GS/GOGAT)循環(huán)均顯呈下降趨勢。而胺化谷氨酸脫氫酶(NADH-GDH,谷氨酸脫氫酶(NAD⁺-GDH,丙氨酸氨基轉(zhuǎn)移酶(AlaAT)以及天冬氨酸氨基轉(zhuǎn)移酶(AspAT)的活性顯著增加�？傊�,與野大豆相比,栽培大豆的生長,光合作用,離子調(diào)節(jié)和氮同化酶的下降幅度更大。栽培大豆對鹽脅迫更加敏感,堿性鹽脅迫的影響高于中性鹽脅迫。野生大豆具有更好的光合作用調(diào)節(jié)機制和硝酸鹽同化機制。本研究結(jié)果能夠為野大豆種...

【文章頁數(shù)】：63 頁

【學(xué)位級別】：碩士

【文章目錄】：
Abstract
摘要
Chapter1:Introduction
    1.1 An overview of Wild and Cultivate soybean
    1.2 Salinity stress
        1.2.1 Types of salinity stress
        1.2.2 Impacts of salinity stress
        1.2.3 Impacts of salinity stress on ions homeostasis
        1.2.4 Impacts of salinity stress on photosynthesis
        1.2.5 Impacts of salinity stress on nitrogen(N)metabolism
    1.3 Chitchat of nitrogen and carbon metabolism
    1.4 Mechanisms of Salinity Tolerance
        1.4.1 Salinity stress and ionic regulation
        1.4.2 Salinity stress and antioxidant defense system
        1.4.3 Salinity Stress and organic compatible solutes
    1.5 Review of Literature
    1.6 The purpose and significance of this study
        1.6.1 Scientific problem
        1.6.2 Aims and contents of the study
        1.6.3 Research theoretical value and practical significance
Chapter2:Materials & Methods
    2.1 Plant materials and salt treatments
    2.2 Growth Parameters
    2.3 Gas Exchange Parameters
    2.4 Measurement of Photosynthetic Pigments
    2.5 Mineral ion content determination
    2.6 Enzyme extracts preparation
        2.6.1 Nitrate reductase(NR)activity
        2.6.2 Glutamine synthetase(GS)activity
        2.6.3 Glutamine synthase(GOGAT)activity
        2.6.4 Glutamate dehydrogenase(NADH-and NAD-GDH)activity
        2.6.5 Aminotransferase(AspAT and AlaAT)activity
        2.6.6 Protein determinations
    2.7 Data Analysis
Chapter3:Results
    3.1 Changes in plant growth performance
    3.2 Analysis of the ionome
        3.2.1 Changes in cation concentrations
        3.2.2 Changes in anion concentrations
    3.3 Changes in photosynthesis parameters
        3.3.1 Changes in photosynthetic pigments
        3.3.2 Changes in photosynthetic gaseous exchange parameters
    3.4 Changes in nitrogen assimilation enzyme activities
        3.4.1 Changes in nitrate reductase(NR)activity
        3.4.2 Changes in glutamine synthetase/glutamine synthase(GS/GOGAT)activities
        3.4.3 Changes in glutamate dehydrogenase and aminotransferases activities
Chapter4:Discussions
Chapter5:Conclusions
Chapter6:References
List of Abbreviation
Acknowledgments



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