High grain protein content（GPC） reduces rice eating and cooking quality（ECQ）. We generated OsAAP6 and OsAAP10 knockout mutants in three high-yielding japonica varieties and one japonica line using the CRISPR/Cas9 system. Mutation efficiency varied with genetic background in the T₀ generation, and GPC in the T₁ generation decreased significantly,owing mainly to a reduction in glutelin content. Amylose content was down-regulated significantly in some Osaap6 and all Osaap10 mu... 

【文章頁數(shù)】：8 頁

【文章目錄】：
1. Introduction
2. Materials and methods
    2.1. Field planting and trait measurement
    2.2. Vector construction and transformation
    2.3. Grain protein content(GPC)measurement
    2.4. Amino acid determination
    2.5. Measurement of pasting properties
    2.6. Taste value measurement
    2.7. RNA extraction and qRT-PCR
    2.8. Statistical analysis
3. Results
    3.1. Generation of OsAAP6 and OsAAP10 mutations
    3.2. Grain protein content(GPC)of the OsAAP6 and OsAAP10mutants
    3.3. Amino acid content in Osaap6 and Osaap10 mutants
    3.4. Starch content and physicochemical properties
    3.5. Taste quality in Osaap6 and Osaap10 mutants
    3.6. Agronomic trait in Osaap6 and Osaap10 mutants
4. Discussion
5. Conclusions
Declaration of competing interest
Appendix A.Supplementary data


【參考文獻(xiàn)】：
期刊論文
[1]臭氧脅迫對稻穗不同部位糙米直鏈淀粉含量和RVA譜特征值的影響[J]. 章燕柳,穆海蓉,邵在勝,王云霞,景立權(quán),王余龍,楊連新.  應(yīng)用生態(tài)學(xué)報. 2019(12)
[2]Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice[J]. Lan Shen,Yufeng Hua,Yaping Fu,Jian Li,Qing Liu,Xiaozhen Jiao,Gaowei Xin,Junjie Wang,Xingchun Wang,Changjie Yan,Kejian Wang.  Science China（Life Sciences）. 2017(05)
[3]Performance and Inheritance of Rice Starch RVA Profile Characteristics[J]. YAN Chang-jie, Li Xin, ZHANG Rong, Sui Jiong-ming, LIANG Guo-hua, SHEN Xin-ping, Gu Shi-liang, Gu Ming-hong (The Key Laboratory of Education Ministry for Plant Functional Genomics, Yangzhou University, Yangzhou 225009, China).  Rice Science. 2005(01)



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