Male sterile genes and mutants are valuable resources in hybrid seed production for monoclinous crops.High genetic redundancy due to allohexaploidy makes it difficult to obtain the nuclear recessive male sterile mutants through spontaneous mutation or chemical or physical mutagenesis methods in wheat.The emerging effective genome editing tool,CRISPR/Cas9 system,makes it possible to achieve simultaneous mutagenesis in multiple homoeoalleles.To improve the genome modification efficiency of the CRI... 

【文章頁數(shù)】：10 頁

【文章目錄】：
1. Introduction
2. Results
    2.1. Ta NP1 genes are preferentially expressed in the tapetum
    2.2. Optimization of the CRISPR/Cas9 system in wheat protoplasts
    2.3. CRISPR/Cas9-mediated mutagenesis of Ta NP1 genes
    2.4. Triple homozygous mutations in Ta NP1 genes result in complete male sterility
    2.5. Any WT copy of the three Ta NP1 genes is sufficient for maintenance of male fertility
3. Discussion
4. Materials and methods
    4.1. Plant materials and growth condition
    4.2. RT-PCR,q RT-PCR,and in situ hybridization
    4.3. Plasmid construction
    4.4. Wheat protoplast isolation and transformation
    4.5. Biolistic-mediated transformation of wheat
    4.6. PCR/RE assay


【參考文獻(xiàn)】：
期刊論文
[1]Research and application of hybrid wheat in China[J]. Zhao Changping.  Engineering Sciences. 2013(05)
[2]C49S溫光型兩系雜種小麥在江漢平原生產(chǎn)應(yīng)用中的幾個問題[J]. 陳功海,龔德平,郭西陵,邱文兵,何慶虎.  麥類作物學(xué)報. 2005(04)
[3]普通小麥D~2型CMS系的育性基因及其遺傳特性[J]. 劉春光,侯寧,劉根齊,吳郁文,張翠蘭,張炎.  遺傳學(xué)報. 2002(07)



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