OsSRK1, an Atypical S-Receptor-Like Kinase Positively Regula
發(fā)布時(shí)間:2022-08-02 10:27
Receptor-like kinases(RLKs) are important for plant growth, development and defense responses. The S-receptor protein kinases(SRKs), which represent an RLK subfamily, control the selfincompatibility among Brassica species. However, little information is available regarding SRK functions in rice. We identified a gene OsSRK1 encoding an atypical SRK. The transcript of OsSRK1 was induced by abscisic acid(ABA), salt and polyethylene glycol. OsSRK1 localized to the plasma membrane and cytoplasm. Leaf...
【文章頁(yè)數(shù)】:10 頁(yè)
【文章目錄】:
MATERIALS AND METHODS
Rice materials and growth conditions
Histological analysis
Reverse transcription polymerase chain reaction(RT-PCR)
Quantitative RT-PCR(qRT-PCR)
Construction of plant expression vector and rice transformation
Production of OsSRK1-green fluorescent protein(GFP)fusion protein
GeneChip analysis
RESULTS
OsSRK1 is an atypical S-receptor-like kinase localized to plasma membrane and cytoplasm
Expression patterns of OsSRK1
Overexpression of OsSRK1 increased leaf width
Overexpression of Os SRK1 enhanced ABA sensitivity and salt tolerance
Genes regulated by OsSRK1
DISCUSSION
Os SRK1 is not a typical S-receptor-like kinase
Overexpression of OsSRK1 increases leaf width probably by promoting cell cycle in leaf primordium
Overexpression of Os SRK1 enhances salt tolerance probably by regulating stress-related genes andABA pathway
SUPPLEMENTAL DATA
【參考文獻(xiàn)】:
期刊論文
[1]水稻蛋白激酶基因啟動(dòng)子OsSRLp的分離及特性分析[J]. 王慶國(guó),王瑩瑩,李臻,潘教文,王一帆,李穎秀,劉煒. 中國(guó)水稻科學(xué). 2018(04)
本文編號(hào):3668352
【文章頁(yè)數(shù)】:10 頁(yè)
【文章目錄】:
MATERIALS AND METHODS
Rice materials and growth conditions
Histological analysis
Reverse transcription polymerase chain reaction(RT-PCR)
Quantitative RT-PCR(qRT-PCR)
Construction of plant expression vector and rice transformation
Production of OsSRK1-green fluorescent protein(GFP)fusion protein
GeneChip analysis
RESULTS
OsSRK1 is an atypical S-receptor-like kinase localized to plasma membrane and cytoplasm
Expression patterns of OsSRK1
Overexpression of OsSRK1 increased leaf width
Overexpression of Os SRK1 enhanced ABA sensitivity and salt tolerance
Genes regulated by OsSRK1
DISCUSSION
Os SRK1 is not a typical S-receptor-like kinase
Overexpression of OsSRK1 increases leaf width probably by promoting cell cycle in leaf primordium
Overexpression of Os SRK1 enhances salt tolerance probably by regulating stress-related genes andABA pathway
SUPPLEMENTAL DATA
【參考文獻(xiàn)】:
期刊論文
[1]水稻蛋白激酶基因啟動(dòng)子OsSRLp的分離及特性分析[J]. 王慶國(guó),王瑩瑩,李臻,潘教文,王一帆,李穎秀,劉煒. 中國(guó)水稻科學(xué). 2018(04)
本文編號(hào):3668352
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