Haynaldia villosa（2 n=14, VV）, a wild grass of the subtribe Triticeae, serves as potential gene resources for wheat genetic improvement. In this study, the proteome characterization during grain development of Yangmai 5 and Yangmai 5-H. villosa 6 VS/6 AL translocation line was investigated by a comparative proteomic approach. Two-dimensional electrophoresis identified 81 differentially accumulated proteins（DAPs） during five grain developmental stages in wheat-H. villosa translocation line. These... 

【文章來源】：Journal of Integrative Agriculture. 2020,19(11)SCICSCD

【文章頁數(shù)】：15 頁

【文章目錄】：
1. Introduction
2. Materials and methods
    2.1. Plant materials and field trial
    2.2. Agronomic trait testing
    2.3. Protein extraction
    2.4.2-DE and image analysis
    2.5. MALDI-TOF/TOF-MS
    2.6. Subcellular localization through website prediction and wheat protoplast transformation
    2.7. Hierarchical clustering analysis
    2.8. Identification of the cis-acting elements in the adverse responsive protein genes
    2.9. RNA-seq expression analysis
    2.1 0. mRNA extraction and RT-qPCR
3. Results
    3.1. Differential proteome identification between Yangmai 5 and 6VS/6AL translocation line
    3.2. Functional classification and subcellular localization
    3.3. Dynamic proteome expression profiling during grain development of 6VS/6AL translocation line
    3.4. Analysis of cis-acting elements in the key DAP genes
    3.5. RNA-seq expression analysis of the DAP genes in different organs and development stages
    3.6. Dynamic transcriptionalexpression profiling of the key DAPgenes during grain development as revealed by RT-qPCR
4. Discussion
    4.1. Biotic stress related proteins from 6VS
    4.2. Abiotic stress related proteins from 6VS
5. Conclusion


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期刊論文
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