干旱和鹽脅迫是影響作物生產(chǎn)布局和產(chǎn)量的重要因素。微生物組被認(rèn)為是植物體的第二套基因組,對(duì)植物的生長(zhǎng)和適逆至關(guān)重要。本課題主要通過對(duì)正常和鹽/干旱脅迫下生長(zhǎng)的棉花根際微生物群進(jìn)行分離和測(cè)序,研究棉花根際微生物的組成,分離對(duì)棉花耐鹽和抗旱有利的微生物種類及研究其功能。主要結(jié)果如下:研究了鹽脅迫條件下棉花植株與PGP根際細(xì)菌之間的相互作用。研究首先進(jìn)行棉花鹽脅迫處理下根際微生物的分離和培養(yǎng),成功獲得了枯草芽孢桿菌（Bacillus subtilis,BB）和短小芽孢桿菌（Bacillus pumilus,BC）等細(xì)菌菌株。然后將這些菌株施加到鹽脅迫處理（200m M Na Cl）的棉花根際,發(fā)現(xiàn)BB和BC處理可以增強(qiáng)棉花對(duì)鹽脅迫的抗性,表現(xiàn)在添加BB或者BC的棉花植株根、莖和葉對(duì)鹽處理敏感性降低。研究進(jìn)一步對(duì)BB或BC處理的鹽脅迫下棉花根系進(jìn)行轉(zhuǎn)錄組測(cè)序。結(jié)果表明,在有或沒有枯草芽孢桿菌（S和BB）的條件下,共有5535個(gè)差異表達(dá)的基因,上調(diào)基因3349個(gè),下調(diào)基因2186個(gè);而在有或沒有短小芽孢桿菌（S和BC）的條件下,DEG是8452個(gè),其中包括4035個(gè)上調(diào)基因和4417下調(diào)基因。GO... 

【文章來源】：華中農(nóng)業(yè)大學(xué)湖北省 211工程院校 教育部直屬院校

【文章頁數(shù)】：102 頁

【學(xué)位級(jí)別】：碩士

【文章目錄】：
摘要
Abstract
List of abbreviations
Chapter 1
    Introduction
    1.1 Cotton
    1.2 Plants Stress
        1.2.1 Salt Stress
        1.2.2.Drought stress
        1.2.3 Drought stress in cotton
        1.2.4 Effect of salinity on plant growth
        1.2.5 Salt stress in cotton
    1.3 PGP Bacteria and their role in abiotic stress
        1.3.1 Role of PGP in controlling salt stress
    1.4 Aims of the thesis
Chapter 2 Salt stress Tolerance conferred by plant growth promoting rhizosphere bacteria in Cotton
    Introduction
    2.1 Materials and Methods
        2.1.1 Identification and selection of salt stress resistant PGP bacteria in Cotton Rhizosphere
            2.1.1.1 Soil preparation
            2.1.1.2 Cotton seed germination,Salt stress and Harvesting
            2.1.1.3 Isolation of bacteria from Rhizosphere,roots and soil
            2.1.1.4 Screening of Bacteria for Salt stress
            2.1.1.5 16SrRNA sequencing and identification of Bacterial strains
        2.1.2 Cotton plants treatment with salt stress and bacteria
            2.1.2.1 Cotton seeds germination and treatments
            2.1.2.2 Phenotype appearance and selection
            2.1.2.3 Sampling and RNA-extraction
            2.1.2.4 Library construction and sequencing
            2.1.2.5 Illumina sequences analysis
            2.1.2.6 Statistical analysis of differentially expressed genes(DEGs)
            2.1.2.7 Gene ontology and pathway enrichment analysis
            2.1.2.8 Quantitative PCR(q PCR)
    2.2 Results
        2.2.1 Bacillus subtilis(BB)and Bacillus pumilus(BC)promoted Cotton growth under salt stress conditions
        2.2.2 Difference shown in leaves numbers and development
        2.2.3 Height variations of Plants
        2.2.4 Root length variations
        2.2.5 Transcriptomic Analysis
            2.2.5.1 Expression density distribution
            2.2.5.2 Correlation between samples
            2.2.5.3 Effects on the expression patterns of DEGs
            2.2.5.4 Gene Ontology enrichment of DEGs
            2.2.5.5 KEGG pathways Analysis
        2.2.6 Selected Pathways for salt stress resistance and Plant growth promotion
            2.2.6.1 Pentose and Glucuronate Interconversions Pathway
            2.2.6.2 Starch and Sucrose Metabolism Pathway
        2.2.7 Validation of DEGs by q RT-PCR
    2.3 Discussion
    2.4 Summary and future prospects
Chapter 3 Microbiome Diversity in Cotton Rhizosphere Under Normal and Drought Conditions
    Introduction
    3.1 Materials and methods
        3.1.1 Plant material and drought treatment
        3.1.2 Sample collection,DNA extraction,and16S r RNA gene amplification
        3.1.3 Illumina library preparation and High-throughput sequencing
        3.1.4 Primary Data processing and Operational Taxonomic Unit demarcation
        3.1.5 Alpha diversity and Taxonomic analysis
        3.1.6 Beta diversity analysis
        3.1.7 Metagenome analysis and network construction
        3.1.8 Statistical analysis
    3.2 Results
        3.2.1 General analysis of sequencing data
        3.2.2 Alpha diversity analysis and Taxonomic analysis
        3.2.3 Beta diversity
        3.2.4 Metagenome prediction
    3.3 Discussion
    3.4 Conclusions
References
Appendices
    Appendix1
    Appendix2
    Appendix3
    Appendix4
    Appendix5
    Appendix6
    Appendix7
List of publications
Acknowledgement


【參考文獻(xiàn)】：
期刊論文
[1]Polyamine Accumulation in Transgenic Tomato Enhances the Tolerance to High Temperature Stress[J]. Lin Cheng,Yijing Zou,Shuli Ding,Jiajing Zhang,Xiaolin Yu,Jiashu Cao and Gang Lu(Key Laboratory of Horticultural Plant Growth,Development and Biotechnology,Ministry of Agriculture,Department of Horticulture,Zhejiang University,Hangzhou 310029,China).  Journal of Integrative Plant Biology. 2009(05)



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