The rice mutant ossac4（starch accumulating 4） was raised from seeds of the rice（Oryza sativa L.） indica maintainer line Xinong 1 B treated with ethyl methanesulfonate. The distal and medial portions of the second leaf displayed premature senescence in the ossac4 mutant at the four-leaf stage. Physiological and biochemical analysis, and cytological examination revealed that the ossac4 mutant exhibited the premature leaf senescence phenotype. At the four-leaf stage, the leaves of the ossac4 mutant... 

【文章來源】：Journal of Integrative Agriculture. 2020,19(09)SCICSCD

【文章頁數(shù)】：15 頁

【文章目錄】：
1. Introduction
2. Materials and methods
    2.1. Plant materials
    2.2. Pigment concentration and photosynthetic parameter measurement
    2.3. Lipid peroxidation and ROS-scavenging enzyme assays
    2.4. Cytological analysis
    2.5. Determination of sugar concentration and iodine-potassium iodide staining in leaves
    2.6. Extraction of total RNA and quantitative real-time PCR analysis
    2.7. Genomic DNA extraction and PCR amplification
    2.8. Genetic analysis and genetic map construction
3. Results
    3.1. Phenotypes and agronomic traits
    3.2. Chloroplast and photosynthetic characteristics
    3.3. Physiological parameters
    3.4. Leaf sugar content analysis
    3.5. Expression of genes related to glucose and photosynthetic metabolism
    3.6. Genetic analysis and genetic map construction
4. Discussion
    4.1. Mutation of Os SAC4 induces leaf starch accumulation
    4.2. Mutation of Os SAC4 severely inhibits chloroplast functioning
    4.3. Mutation of Os SAC4 induces premature leaf senescence
5. Conclusion


【參考文獻】：
期刊論文
[1]水稻葉片淀粉積累及早衰突變體esl9的鑒定與基因定位[J]. 肖艷華,陳新龍,杜丹,邢亞迪,張?zhí)烊?祝毛迪,劉明明,朱小燕,桑賢春,何光華.  作物學報. 2017(04)



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