Genetic analysis and QTL mapping of a novel reduced height g
發(fā)布時間:2021-10-26 07:12
Low stature in wheat is closely associated with lodging resistance, and this impacts harvest index and grain yield. The discovery of novel dwarfing or semi-dwarfing genes can have great significance for dwarf wheat breeding. In this study, we identified an EMS-induced dwarf wheat mutant JE0124 from the elite cultivar Jing411. JE0124 possesses increased stem strength and a 33% reduction in plant height compared with wild type. Gibberellic acid(GA) treatment analysis suggested that JE0124 was GA-s...
【文章來源】:Journal of Integrative Agriculture. 2020,19(07)SCICSCD
【文章頁數(shù)】:10 頁
【文章目錄】:
1. Introduction
2. Materials and methods
2.1. Plant materials and growth measurement
2.2. Cytological and histological analysis
2.3. GA treatment
2.4. DNA extraction and quality assessment
2.5. Bulked segregant analysis(BSA)by using wheat660K SNP array
2.6. Development of molecular markers by kompetitive allele-specific PCR(KASP)assays
2.7. Linkage map construction
3. Results
3.1. Phenotypic variations of the dwarf mutant JE0124
3.2. Internode characteristics and GA sensitiveness
3.3. Genetic analysis of reduced height gene in JE0124
3.4. Mapping QTL using 660K SNP array-based BSA
3.5. Genetic linkage map analysis of the Rht gene on chromosome 2DL
4. Discussion
5. Conclusion
【參考文獻(xiàn)】:
期刊論文
[1]用STS標(biāo)記檢測矮稈基因Rht-B1b和Rht-D1b在中國小麥中的分布[J]. 楊松杰,張曉科,何中虎,夏先春,周陽. 中國農(nóng)業(yè)科學(xué). 2006(08)
本文編號:3459078
【文章來源】:Journal of Integrative Agriculture. 2020,19(07)SCICSCD
【文章頁數(shù)】:10 頁
【文章目錄】:
1. Introduction
2. Materials and methods
2.1. Plant materials and growth measurement
2.2. Cytological and histological analysis
2.3. GA treatment
2.4. DNA extraction and quality assessment
2.5. Bulked segregant analysis(BSA)by using wheat660K SNP array
2.6. Development of molecular markers by kompetitive allele-specific PCR(KASP)assays
2.7. Linkage map construction
3. Results
3.1. Phenotypic variations of the dwarf mutant JE0124
3.2. Internode characteristics and GA sensitiveness
3.3. Genetic analysis of reduced height gene in JE0124
3.4. Mapping QTL using 660K SNP array-based BSA
3.5. Genetic linkage map analysis of the Rht gene on chromosome 2DL
4. Discussion
5. Conclusion
【參考文獻(xiàn)】:
期刊論文
[1]用STS標(biāo)記檢測矮稈基因Rht-B1b和Rht-D1b在中國小麥中的分布[J]. 楊松杰,張曉科,何中虎,夏先春,周陽. 中國農(nóng)業(yè)科學(xué). 2006(08)
本文編號:3459078
本文鏈接:http://sikaile.net/nykjlw/nzwlw/3459078.html
最近更新
教材專著