The functional diversity of plant valine-qlutamine（VQ） proteins is closely associated with their partners WRKY transcription factors, and also with a complex network of signaling pathways that mediated by hormone molecules. We reported genome-wide expression profiles of differentially expressed rice VQ genes under nitric oxide（NO） treatment based on a microarray analysis. Cluster analysis of expression patterns revealed that some VQ genes and WRKY genes shared similar expression trends. Predicti... 

【文章來源】：Rice Science. 2020,27(01)SCICSCD

【文章頁數(shù)】：11 頁

【文章目錄】：
MATERIALS AND METHODS
    Plant materials and NO treatment
    RNA isolation,microarray hybridization and data process
    Quantitative real-time PCR(qRT-PCR)analysis
    Analysis for cis-elements of promoters
    Transient expression assay in tobacco cells
    Chromatin immunoprecipitation(ChIP)assay
    Yeast two-hybrid assay
RESULTS
    Some VQ genes were differentially expressed under NO treatment
    W-box,or W-box like cis-elements were enriched in promoters of differentially expressed VQ genes
    Some VQ genes showed similar expression patterns with WRKY genes in response to NO treatment
    OsWRKY24 binded to promoters of Os VQ7 and Os WRKY24 itself
    OsVQ7 interacted physically with OsWRKY24through C-terminal WRKY domain
DISCUSSION
    VQ genes may be involved in NO signalingmediated physiological processes
    Similar expression of WRKY and VQ genes suggests their possible interactions during regulatory processes
    Could OsVQ7 and OsWRKY24 module play a role in regulating plant immunity?
SUPPLEMENTAL DATA


【參考文獻】：
期刊論文
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[2]水稻W(wǎng)RKY轉(zhuǎn)錄因子基因家族響應外源一氧化氮的表達譜分析[J]. 孟姣,王海華,向建華,蔣丹,彭喜旭,賀歡歡.  中國水稻科學. 2016(02)
[3]擬南芥VQ基因家族響應抗性相關激素表達譜分析[J]. 林淵源,余迪求.  植物分類與資源學報. 2012(05)



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