The efficiency of plant cytidine base-editing systems is limited, and unwanted mutations frequently occur in transgenic plants. We increased the cytidine editing frequency and fidelity of the plant base editor 3（BE3） and targeted activation-induced cytidine deaminase（CDA）（target-AID） systems by coexpressing three copies of free uracil–DNA glycosylase（UDG） inhibitor（UGI）. The editing efficiency of the improved BE3 and CDA systems reached as high as 88.9% and 85.7%, respectively, in regenerated ri... 

【文章來源】：The Crop Journal. 2020,8(03)SCICSCD

【文章頁數(shù)】：7 頁

【文章目錄】：
1. Introduction
2. Materials and methods
    2.1. Vector construction
    2.2. Rice transformation
    2.3. DNA extraction and genotyping
3. Results
4. Discussion
Declaration of Competing Interest


【參考文獻】：
期刊論文
[1]Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems[J]. Qing Liu,Chun Wang,Xiaozhen Jiao,Huawei Zhang,Lili Song,Yanxin Li,Caixia Gao,Kejian Wang.  Science China（Life Sciences）. 2019(01)
[2]A CRISPR/Cas9 toolkit for efficient targeted base editing to induce genetic variations in rice[J]. Bin Ren,Fang Yan,Yongjie Kuang,Na Li,Dawei Zhang,Honghui Lin,Huanbin Zhou.  Science China（Life Sciences）. 2017(05)
[3]CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis[J]. Yiyu Chen,Zhiping Wang,Hanwen Ni,Yong Xu,Qijun Chen,Linjian Jiang.  Science China（Life Sciences）. 2017(05)
[4]Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice[J]. Rongfang Xu,Yachun Yang,Ruiying Qin,Hao Li,Chunhong Qiu,Li Li,Pengcheng Wei,Jianbo Yang.  Journal of Genetics and Genomics. 2016(08)



本文編號：3209524