Waxy maize is a specialty maize that produces mainly amylopectin starch with special food or industrial values. The objective of this study was to overcome the limitations of wx mutant allele acquisition and breeding efficiency by conversion of parental lines from normal to waxy maize. The intended mutation activity was achieved by in vivo CRISPR/Cas9 machinery involving desired-target mutation of the Wx locus in the ZC01 background,abbreviated as ZC01-DTM^wx. Triple selection was appl... 

【文章來源】：The Crop Journal. 2020,8(03)SCICSCD

【文章頁數(shù)】：9 頁

【文章目錄】：
1. Introduction
2. Materials and methods
    2.1. In vivo desired-target-mutation(DTM)donor and creation of the mutation
    2.2. Detection of genetically modified component
    2.3. Sanger sequencing to identify the target mutation
    2.4. Mutant phenotype verification in endosperm starch and pollen grains
    2.5. Measurement of amylopectin content
    2.6. Whole-genome SNP genotyping and genome background recovery estimation
    2.7. Field experiment and trait measurements
3. Results
    3.1. The experimental strategy and rationale for the experi-mental design
    3.2. Generation of desirable ZmWX mutations in two parent recipient lines of a hybrid
    3.3. Identification of transgene-free edited lines and validation of the mutant phenotypes
    3.4. Genome background recoveries of the mutant lines
    3.5. Agronomic performance comparison between the wild type and the created mutants
4. Discussion
    4.1. Mutant wx alleles in conversion lines were targeted by DTMwxbut not mutant allele introgression
    4.2. Creation of mutant parental lines with different genome recovery proportions
    4.3. Agronomic performance of the mutants and the wild type
Declaration of competing interest
Author contributions



本文編號：3174803