發(fā)布時間：2024-02-22 20:44

　　本研究的目的是為了解決種子在基因庫標(biāo)準(zhǔn)和低溫儲藏下的一些關(guān)鍵問題,主要包括休眠和水分含量兩個方面的研究。盡管在低溫貯藏中,種子的休眠和水分含量是可控的,但是對于長期保存還是比較難控制的。第2章主要研究車桑子種子的物理休眠和低溫保存的可行性。更詳細(xì)地說是研究在種子保存過程中何時和用哪種方式解除物理休眠。第3章將第2章的內(nèi)容擴(kuò)展到杏仁種子的研究中,該部分的研究可以應(yīng)用于其他薔薇科物種和具有堅實內(nèi)果皮的種子。第4和5章主要研究了萵苣種子的貯藏,為其他異常型種子的保存提供了借鑒。第4章主要從降溫速率上切入優(yōu)化了種子的保存條件。第5章主要研究了低溫保護(hù)劑對種子保存的影響。主要內(nèi)容如下:本章將對種子的物理休眠和低溫貯藏的可行性進(jìn)行研究。采用車桑子(Sapindaceae)種子為研究對象,該種子具有不透水的種皮且進(jìn)行物理休眠。目前,已有的研究可以在實驗室的條件下可以對種子進(jìn)行機(jī)械劃痕、干燥、硫酸和熱水處理來改變種子的通透性從而解讀種子的休眠釋放機(jī)理,但是在自然條件下該機(jī)理還未被完全解讀。本章的主要研究內(nèi)容是理解車桑子種子在人工填埋2年后的休眠釋放機(jī)理。此外,本文還設(shè)計了種子的低溫貯藏程序。新鮮的種子...

【文章頁數(shù)】：113 頁

【學(xué)位級別】：博士

【文章目錄】：
摘要
ABSTRACT
Abbreviations
Chapter 1 Introduction
    1.1 SEED STORAGE
        1.1.1 Types of seeds
        1.1.2 Usefulness of discrete classification system
        1.1.3 Seed storage conditions
        1.1.4 Selecting and treating seeds for storage
        1.1.5 Drying seeds – importance of drying rate
        1.1.6 Cryoprotectants
    1.2 FREEZING OF IMBIBED SEEDS IN NATURAL ENVIRONMENT
        1.2.1 Freezing avoidance-supercooling
        1.2.2 Freezing tolerance by freeze-desiccation
    1.3 STATE OF WATER IN SEEDS
    1.4 USING ORTHODOX SEEDS AT IMBIBED STATE AS A MODEL FOR RECALCITRANT SPECIES
    1.5 DORMANCY
    1.6 OUTLINE OF THE CURRENT PROJECT
Chapter 2 Dormancy and cryopreservation of Dodonea viscosa seeds
    2.1 INTRDOUCTION
    2.2 MATERIALS AND METHODS
        2.2.1 Seed collection
        2.2.2 Moisture content measurement
        2.2.3 Germination test
        2.2.4 Dormancy breaking
        2.2.5 Seed imbibition
        2.2.6 Cryopreservation
        2.2.7 Statistical analysis
    2.3 RESULTS
        2.3.1 Germination and moisture content of freshly collected seed
        2.3.2 Seed imbibition
        2.3.3 Effect of dry storage on dormancy breaking
        2.3.4 Effect of different temperatures on breaking dormancy
        2.3.5 Importance of hot-water temperature in breaking dormancy
        2.3.6 Cryopreservation of seeds
    2.4 DISCUSSION
Chapter 3 Dormancy and cryopreservation of Apricot (Prunus armeniaca) seeds fromXinjiang province
    3.1 INTRODUCTION
    3.2 MATERIALS AND METHODS
        3.2.1 Seed materials
        3.2.2 Seed desiccation and moisture content determination
        3.2.3 Germination test
        3.2.4. Imbibition of water
        3.2.5 Cold stratification
        3.2.6 Effect of GA3 on dormancy break
        3.2.7 Effect of GA3 in endocarp intact seeds
        3.2.8 Cryopreservation of seeds
        3.2.9 Statistical analysis
    3.3 RESULTS
        3.3.1 Moisture content of the seeds
        3.3.2 Germination of seeds
        3.3.3 Imbibition of water
        3.3.4 Effect of GA3 on dormancy break
        3.3.5 Effect of GA3 in endocarp intact seeds
        3.3.6 Cold stratification
        3.3.7 Seed desiccation and germination
        3.3.8 Cryopreservation of seeds
    3.4 DISCUSSION
Chapter 4 Cryopreservation of hydrated lettuce (Lactuca sativa L.) seeds
    4.1 INTRODUCTION
    4.2 MATERIALS AND METHODS
        4.2.1 Seed material and germination conditions
        4.2.2 Seed imbibition and moisture content determination
        4.2.3 Thermal analyses
        4.2.4 Controlled cooling of seeds
        4.2.5 Liquid nitrogen storage
        4.2.6 Use of pierced seeds
        4.2.7 Statistical analysis
    4.3 RESULTS
    4.4 DISCUSSION
Chapter 5 Effects of dimethyl sulfoxide concentration, pre-cooling and cooling rate oncryopreservation of hydrated lettuce seeds
    5.1 INTRODUCTION
    5.2 MATERIALS AND METHODS
        5.2.1 Seed materials
        5.2.2 Seed germination
        5.2.3 Moisture content assessment
        5.2.4 Seed imbibition
        5.2.5 DMSO treatment
        5.2.6. Freezing treatments and differential thermal analysis (DTA)
        5.2.7 Statistical analysis
    5.3 RESULTS
    5.4. DISCUSSION
Chapter 6 Summary of main findings
References
List of papers published or in progress duringthe post graduate study
Acknowledgements



本文編號：3907240