赤皮青岡（Cyclobalnopsis gilva）屬于殼斗科植物,原產(chǎn)于中國和日本等東南亞國家。赤皮青岡是一種優(yōu)良的硬木樹種,但是其種子發(fā)芽率低和萌發(fā)整齊度較差。應(yīng)用RNA-seq、2-DE和Label-free等方法探討在mRNA和蛋白水平上赤皮青岡種子萌發(fā)的分子生理機(jī)制。應(yīng)用Illumina HiSeq 2500平臺(tái)對(duì)赤皮青岡種子的轉(zhuǎn)錄組進(jìn)行分析。通過匹配Nr、SwissProt、GO和KEEG等公共數(shù)據(jù)庫上序列,結(jié)果顯示54,130條unigene被注釋。應(yīng)用GO和KEGG pathway數(shù)據(jù)庫分析這些unigene,結(jié)果發(fā)現(xiàn)許多unigene富集于淀粉和蔗糖代謝、糖酵解、植物激素信號(hào)轉(zhuǎn)導(dǎo)、ABC轉(zhuǎn)運(yùn)子、碳水化合物代謝、翻譯后修飾以及核糖體結(jié)構(gòu)與生物合成等生物學(xué)過程。這些轉(zhuǎn)錄組數(shù)據(jù)表明許多種子萌發(fā)基因可以作為參考基因數(shù)據(jù)庫用于分析赤皮青岡種子萌發(fā)過程基因的差異表達(dá)。萌發(fā)種子與未萌發(fā)種子間的差異基因的分析結(jié)果顯示26,003條unigenes被鑒定出在兩樣本間表達(dá)差異達(dá)2倍以上,其中在萌發(fā)種子中有9683條unigenes上調(diào)表達(dá),16,320條unigenes下調(diào)表達(dá)。未萌發(fā)種... 

【文章頁數(shù)】：117 頁

【學(xué)位級(jí)別】：博士

【文章目錄】：
Abbreviations
中文摘要
ABSTRACT
1 Introduction
    1.1 Physiological features in seed germination
    1.2 Transcriptomic approach to address seed germination
        1.2.1 Energy production for germination
        1.2.2 Genes involve in starch and sucrose metabolism
        1.2.3 Genes involve in protein mobilization
        1.2.4 Activation of developmental genes
        1.2.5 Defense genes activation
        1.2.6 Phytohormones in seed germination
    1.3 Proteomic approach to address seed germination
        1.3.1 Proteins involved in regulating germination
        1.3.2 Progress in proteomics related to seed germination
        1.3.3 Proteins involved in seed germination
    1.4 Transcriptome and proteomics interaction
        1.4.1 Transcription and translation activation
        1.4.2 Translation role in seed germination
        1.4.3 Comparison of the seed transcriptome and proteome
        1.4.4 Post-translational control of seed germination
    1.5 Objectives and technical route
        1.5.1 Objectives
        1.5.2 Technical route
2 Transcriptome analysis in Cyclobalnopsis gilva seed germination
    2.1 Materials and methods
        2.1.1 Plant materials
        2.1.2 RNA extraction and construction of cDNA libraries for transcriptomesequencing
        2.1.3 Illumina sequencing, assembly and annotation
        2.1.4 Analysis of quantitative real-time PCR (qRT-PCR)
        2.1.5 Analysis of physiological parameters
        2.1.6 Statistical analysis
    2.2 Results
        2.2.1 Sequence analysis and assembly from C. gilva seeds
        2.2.2 Analysis of differentially expressed unigenes (DEGs) by RNA-seq
        2.2.3 DEGs validation during seed germination
        2.2.4 Physiological profiles during seed germination
    2.3 Discussion
        2.3.1 Transcriptional responses in the glycolysis and TCA cycle pathways
        2.3.2 Transcriptional responses in starch and sucrose metabolism
3 Proteomic study on Cyclobalnopsis gilva seed germination
    3.1 Proteomic 2-DE approach to address low germination rate in C. gilva seeds
        3.1.1 Materials and methods
        3.1.2 Results
        3.1.3 Discussion
    3.2 Label-Free quantitative proteomics analysis in C. gilva seed germination
        3.2.1 Materials and methods
        3.2.2 Results
        3.2.3 Discussion
4 Conclusion
Reference
Publication
Acknowledgement


【參考文獻(xiàn)】：
期刊論文
[1]Gene Expression Profile Changes in Germinating Rice[J]. Dongli He1,Chao Han1,2 and Pingfang Yang1 1Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,China 2Graduate University of Chinese Academy of Sciences,Beijing 100049,China.  Journal of Integrative Plant Biology. 2011(10)



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