【摘要】：以D-甘露糖和伴刀豆球蛋白(ConA)的相互作用為研究對象,利用美拉德反應將D-甘露糖共價結(jié)合到負載蛋白牛血清蛋白(BSA)的表面形成擬糖蛋白,然后將擬糖蛋白固定到玻碳電極表面,以擬糖蛋白表面的D-甘露糖為分子識別物質(zhì),構(gòu)建了檢測伴刀豆球蛋白(ConA)的電化學阻抗傳感器。擬糖蛋白制備過程簡單,D-甘露糖負載量大,在空間中提供多個結(jié)合位點,因此能與ConA形成多價復合物,提高了傳感器的靈敏度。該傳感器的響應值與ConA濃度的對數(shù)在5.0×10-11~5.0×10-9mol/L之間呈良好的線性關(guān)系,檢出限為1.7×10-11mol/L,D-甘露糖和ConA之間的結(jié)合常數(shù)為2.6×106L/mol。該方法簡單,可適用于不同糖和蛋白質(zhì)相互作用的研究,為構(gòu)建高靈敏度的電化學阻抗傳感器提供了新思路。
[Abstract]:The interaction between D-mannose and concanavalin (ConA) was studied. D-mannose covalent binding to the surface of loaded protein bovine serum protein (BSA) was carried out by Maillard reaction to form glycosylated protein. Then the glycosylated protein was immobilized on the surface of glassy carbon electrode, and the electrochemical impedance sensor for the detection of concanavalin (ConA) was constructed using D-mannose on the surface of glycosylated protein as molecular recognition material. The preparation process of glycosylated protein is simple, the loading of D-mannose is large, and it provides many binding sites in space, so it can form multivalent complex with ConA and improve the sensitivity of the sensor. The linear relationship between the response value of the sensor and the logarithm of ConA concentration was found in the range of 5.0 脳 10 ~ (-11) mol / L ~ 5.0 脳 10-9mol/L. The detection limit was 1.7 脳 10 ~ (-11) mol / L ~ (-1) -mannose and the binding constant between ConA and ConA was 2.6 脳 10 ~ (-6) L / mol. The method is simple and suitable for the study of the interaction between different carbohydrates and proteins, and provides a new idea for the construction of electrochemical impedance sensors with high sensitivity.
【作者單位】： 寧夏師范學院化學化工學院;固原市原州區(qū)黃鐸堡學校;
【基金】：寧夏師范學院重點項目(NX SFZD1602) 六盤山工程中心項目(HG16-06)
【分類號】：O657.1;TP212.3

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