全場高分辨生物組織光學(xué)層析成像
發(fā)布時(shí)間：2018-09-17 06:34
【摘要】：建立了一套全場光學(xué)相干層析(FFOCT)系統(tǒng),以實(shí)現(xiàn)對(duì)生物組織和細(xì)胞的高分辨層析成像。該光學(xué)系統(tǒng)基于Linnik干涉顯微結(jié)構(gòu)。不同于現(xiàn)有FFOCT系統(tǒng)采用光纖束照明方式,采用鹵鎢燈照明和大數(shù)值孔徑顯微物鏡成像,壓電陶瓷(PZT)移相,面陣電荷耦合器件(CCD)采集干涉信號(hào),由5步移相算法獲取層析圖,最終合成三維圖像。對(duì)整套系統(tǒng)的性能進(jìn)行了詳細(xì)的闡述與分析,并通過對(duì)洋蔥表皮細(xì)胞和盆栽樹葉的光學(xué)層析實(shí)驗(yàn),驗(yàn)證了本系統(tǒng)的可行性和精確度。通過對(duì)集成電路內(nèi)部芯片(英特爾奔騰4,橫向分辨率達(dá)0.8μm)成像,證明了該系統(tǒng)的分辨率可達(dá)到0.7μm×0.5μm(橫向×縱向)。提出的系統(tǒng)分辨率高、成本低、結(jié)構(gòu)簡單便于調(diào)節(jié),為實(shí)現(xiàn)高分辨率光學(xué)相干層析成像提供了簡單易行的方法。
[Abstract]:A set of full-field optical coherence tomography (FFOCT) system was established to realize high resolution tomography of biological tissues and cells. The optical system is based on Linnik interference microstructure. Different from the existing FFOCT system, the optical fiber beam illumination is used, the Halogen-tungsten lamp illumination and the large numerical aperture microscopic objective imaging are used, the piezoelectric ceramic (PZT) phase shift, the plane charge coupled device (CCD) to collect the interference signal, and the five-step phase shift algorithm to obtain the chromatographic image. Finally, three-dimensional images are synthesized. The performance of the whole system was described and analyzed in detail. The feasibility and accuracy of the system were verified by optical chromatography of onion epidermis cells and potted leaves. By imaging the integrated circuit internal chip (Intel Pentium 4, lateral resolution 0.8 渭 m), it is proved that the resolution of the system can reach 0.7 渭 m 脳 0.5 渭 m (lateral 脳 longitudinal). The proposed system has the advantages of high resolution, low cost, simple structure and easy adjustment, which provides a simple and practical method for the realization of high resolution optical coherence tomography.
【作者單位】： 南京理工大學(xué)電光學(xué)院光學(xué)工程系;
【基金】：國家自然科學(xué)基金(61275198、60978069) 國防基礎(chǔ)科研資助項(xiàng)目
【分類號(hào)】：TN911.7
本文編號(hào)：2245014
[Abstract]:A set of full-field optical coherence tomography (FFOCT) system was established to realize high resolution tomography of biological tissues and cells. The optical system is based on Linnik interference microstructure. Different from the existing FFOCT system, the optical fiber beam illumination is used, the Halogen-tungsten lamp illumination and the large numerical aperture microscopic objective imaging are used, the piezoelectric ceramic (PZT) phase shift, the plane charge coupled device (CCD) to collect the interference signal, and the five-step phase shift algorithm to obtain the chromatographic image. Finally, three-dimensional images are synthesized. The performance of the whole system was described and analyzed in detail. The feasibility and accuracy of the system were verified by optical chromatography of onion epidermis cells and potted leaves. By imaging the integrated circuit internal chip (Intel Pentium 4, lateral resolution 0.8 渭 m), it is proved that the resolution of the system can reach 0.7 渭 m 脳 0.5 渭 m (lateral 脳 longitudinal). The proposed system has the advantages of high resolution, low cost, simple structure and easy adjustment, which provides a simple and practical method for the realization of high resolution optical coherence tomography.
【作者單位】： 南京理工大學(xué)電光學(xué)院光學(xué)工程系;
【基金】：國家自然科學(xué)基金(61275198、60978069) 國防基礎(chǔ)科研資助項(xiàng)目
【分類號(hào)】：TN911.7
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1 趙會(huì)娟,高峰,山田幸生;基于多通道時(shí)間分辨光學(xué)層析成像系統(tǒng)的差分圖像重建[J];光學(xué)學(xué)報(bào);2001年12期
，本文編號(hào)：2245014
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