本文選題：漆酶 + 固定化��； 參考：《中國(guó)石油大學(xué)(華東)》2015年碩士論文

【摘要】：在我國(guó)石油煉化企業(yè)中,普遍存在著油品泄漏問題。油品泄漏導(dǎo)致循環(huán)水中含有大量石油烴類物質(zhì),從而影響生產(chǎn)裝置的正常運(yùn)行。生物酶是一種綠色環(huán)保生物催化劑,可以有效的降解油類物質(zhì)。針對(duì)游離漆酶易受環(huán)境條件影響變性失活且反應(yīng)體系不容易分離回收等缺點(diǎn),本文采用固定化漆酶替代游離酶,克服了游離酶的不足,并研究了漆酶的固定化以及在柴油泄漏的循環(huán)水中的除油效果。主要內(nèi)容包括:(1)對(duì)包埋法、交聯(lián)法和包埋-交聯(lián)法這三種固定化酶的方法進(jìn)行了研究,確定了固定化的最優(yōu)條件;(2)優(yōu)化了處理含柴油循環(huán)冷卻水的工藝條件;(3)研究了常規(guī)緩蝕劑PASP與ATMP與固定化漆酶的協(xié)同作用;(4)考察了將固定化漆酶復(fù)合藥劑與活性炭共同制成濾柱,在實(shí)際應(yīng)用時(shí)的除油率。實(shí)驗(yàn)結(jié)果表明:采用交聯(lián)法的固定化漆酶的除油效果最好,最大能降解77.2%的柴油,且固定化酶反復(fù)使用6次時(shí),除油率仍能達(dá)到44%。將常規(guī)緩蝕劑中緩蝕效果較好的ATMP與漆酶共同固定在殼聚糖載體上,對(duì)固定化漆酶緩蝕性能進(jìn)行優(yōu)化,固定化漆酶的緩蝕率從16.22%增加到46.89%。固定化漆酶復(fù)合藥劑與活性炭一起填充到濾柱中,泵流量為0.9L/h,反復(fù)通過濾柱5次除油率為92.8%,裝有等量活性炭的濾柱除油率為30%,從而證明固定化漆酶濾柱除油的可行性。
[Abstract]:Oil leakage is a common problem in petroleum refining and chemical enterprises in China. Oil leakage leads to a large amount of petroleum hydrocarbons in circulating water, which affects the normal operation of the production plant. Bioenzyme is a kind of green biocatalyst, which can degrade oil effectively. In this paper, the immobilized laccase is used to replace the free laccase, which is easy to be deactivated and deactivated under the influence of environmental conditions, and the reaction system is not easy to separate and recover, which overcomes the deficiency of free laccase. The immobilization of laccase and the deoiling effect of laccase in diesel-leaking circulating water were studied. The main contents are as follows: (1) the three immobilized enzyme methods, including entrapment method, crosslinking method and entrapping crosslinking method, were studied. The optimum conditions of immobilization were determined; (2) the process conditions of treating the circulating cooling water containing diesel oil were optimized; (3) the synergistic action of conventional corrosion inhibitor PASP and ATMP with immobilized laccase was studied; (4) the combination of immobilized laccase and its activity was investigated. Carbon is used together to form filter columns, Oil removal rate in practical application. The results showed that the immobilized laccase with crosslinking method had the best deoiling effect, and the maximum degradation rate of diesel oil was 77.2%. When the immobilized laccase was used repeatedly for 6 times, the oil removal rate could still reach 44%. The inhibition rate of the immobilized laccase was increased from 16.22% to 46.89 by immobilization of ATMP and laccase on chitosan carrier. The immobilized laccase compound agent was filled into the filter column together with activated carbon, the pump flow rate was 0.9L / h, the oil removal rate was 92.8 through the filter column 5 times repeatedly, and the oil removal rate of the filter column containing the same amount of activated carbon was 30%, which proved the feasibility of the immobilized laccase filter column oil removal.
【學(xué)位授予單位】：中國(guó)石油大學(xué)(華東)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：Q814.2;TE62

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 盧憲輝;劉芳;陸津津;仲慧，

本文編號(hào)：2090295