本文關(guān)鍵詞：高效石油烴降解菌群的構(gòu)建及其在生物修復中的強化作用研究，由筆耕文化傳播整理發(fā)布。

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所有學科

石油天然氣工業(yè)

環(huán)境科學與資源利用

生物學

海洋學

    Applied Potentials and Living Characters of Hydrocarbon Degradation Bacteria in High-Temperature and High-Salinity Environment

    石油烴降解菌在高溫高鹽環(huán)境中的生理特性及應用潛力

短句來源

    Basic theories, basic method, applied range, merits and defects of 5 methods including microscopic examination, turbidimetric determination, plate-counter method, most probaber number test, culture flask test which are used to measure the bacteria cell population of the hydrocarbon degradation bacteria were reviewed in this paper.

    對石油烴降解菌菌數(shù)常用的 5種測定方法的基本原理、基本方法、適用范圍及優(yōu)缺點進行了評述。

短句來源

    In addition, a series of results have been obtained in relevant theory of hydrocarbon degradation and mathematic model, too.

    相關(guān)的石油烴降解理論、數(shù)學模型等也取得了系列成果。

短句來源

    PCR and the techniques related to genetic engineering will contribute to the construction of multi-function fungus for hydrocarbon degradation and MEOR improvement.

    PCR及基因工程相關(guān)技術(shù)的應用,對構(gòu)建復合性多功能石油烴降解菌及促進MEOR發(fā)展具有重要作用。

短句來源

    Analysis of characteristics of hydrocarbon degrading bacteria in enhanced oil recovery

    石油烴降解菌的特性分析

短句來源

    Determination of Bacterial Cell Population for Hydrocarbon-Degrading Bacteria by Using Culture Flask Test

    細菌瓶法用于石油烴降解菌菌數(shù)測定

短句來源

    THE COMMENT ON THE MEASURING WAYS OF THE BECTERIAL CELL POPULATION OF THE HYDROCARBON BACTERIA

    石油烴降解菌菌數(shù)測定方法評述

短句來源

    Study on Construction of High-Effective Microbiol Consortium for Degrading Petroleum and the Bioaugmentation on Bioremediation

    高效石油烴降解菌群的構(gòu)建及其在生物修復中的強化作用研究

短句來源

    Study on Construction of Bioaugmentation Degradative System and Degradation Capability for Hydrophobic Hydrocarbon Degrading Bacteria

    疏水性石油烴降解菌強化降解系統(tǒng)的構(gòu)建及其降解能力研究

短句來源

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  hydrocarbon degradation

Hydrocarbon degradation in tridecane, decane, and octane emulsions by the action of anodic microdischarge (carbonate electrolyte, AMg-6 aluminum alloy anode, voltage 600 V, current 50-100 mA) was studied.

Hydrocarbon degradation potential of autochthonous bacteria from the Yellow River delta soil

In contrast to the original strain in which hydrocarbon degradation is linked to enzymatic induction mechanisms, the "variant" 101 behaves like a constitutive strain for n-tetradecane.

Estimating the thermodynamics and kinetics of chlorinated hydrocarbon degradation

Media made up with seawater or salts solution supplemented with nitrate and phosphate were employed in the degradation studies; significant growth and hydrocarbon degradation was observed.

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  degradation of petroleum hydrocarbon

The treatment (E) gave the highest number of leaves, % crop emergence, plant biomass, microbial population and degradation of petroleum hydrocarbon compared to any of the treatments that had received crude oil.

  degradation of petroleum hydrocarbon

The treatment (E) gave the highest number of leaves, % crop emergence, plant biomass, microbial population and degradation of petroleum hydrocarbon compared to any of the treatments that had received crude oil.

In the researches, bacterial culture inspection and field trial monitoring of MEOR and in invironmental protection, the main method of determining bacterial cell population for hydrocarbon degrading bacteria(HDB) used today is still time consuming and troublesome the most probable number(MPN) tests. For this a simple and convenient culture flask test is recommended. The flasks containing hydrocarbons (as only carbon source) and proper nitrogen and phosphore sources in cultures as well as a special indicator...

In the researches, bacterial culture inspection and field trial monitoring of MEOR and in invironmental protection, the main method of determining bacterial cell population for hydrocarbon degrading bacteria(HDB) used today is still time consuming and troublesome the most probable number(MPN) tests. For this a simple and convenient culture flask test is recommended. The flasks containing hydrocarbons (as only carbon source) and proper nitrogen and phosphore sources in cultures as well as a special indicator for growth of HDB bacteria. The aqueous samples under test are injected into a series of flasks and diluted successively, step by step, and cultivated at 30℃ for 5-7 days. The change of color of test samples from red to yellow denotes the growth of HDB bacteria. The bacterial cell population is determined by the same procedures as in common flask test. By using the recommended flask test, the bacterial cell population of HDB bacteria in an oilfield produced fluid and an oily sewege, and a bacteria culture for microbial oil recovery at an oil field is determined and practically the same results are obtained as compared with that by MNP test. The culture flasks for HDB bacteria are now manufactured as commercial articles of type HDB JH.

目前在MEOR研究、菌液檢測、現(xiàn)場試驗監(jiān)控及環(huán)保領(lǐng)域 ,石油烴降解菌 (HDB)菌數(shù)的測定基本上采用費時費事的最大概率數(shù)法 (MPN法 )。本文推薦使用簡單易行的細菌瓶法。研制了盛有唯一碳源石油烴、氮源、磷源及專用生長指示劑的培養(yǎng)液 ,適用于HDB菌的測試瓶。欲測水樣注入測試瓶逐級稀釋 ,在 30℃培養(yǎng) 5— 7天。試液由紅變黃表示有HDB菌生長。HDB菌的計數(shù)按常規(guī)細菌瓶法進行。用所推薦的細菌瓶法測定了某油田采出液、污水及采油用菌液中HDB菌數(shù) ,所得結(jié)果與MPN法基本相同。HDB菌測試瓶已有工業(yè)產(chǎn)品 (HDB JH型 )。

Biological technology especially microbial enhanced oil recovery technology attracts the tremendous attention of the petrole-um engineers. And some pilot tests of MEOR have displayed it's talent for the first time at home and abroad. The typical example is in America and Russia. Jilin and Shengli oilfield in China have also taken pilot tests and the effect of incremental oil is inspiring. At the same time, with the development of science and technology, some advanced study tools of microbiology slieti as molecular...

Biological technology especially microbial enhanced oil recovery technology attracts the tremendous attention of the petrole-um engineers. And some pilot tests of MEOR have displayed it's talent for the first time at home and abroad. The typical example is in America and Russia. Jilin and Shengli oilfield in China have also taken pilot tests and the effect of incremental oil is inspiring. At the same time, with the development of science and technology, some advanced study tools of microbiology slieti as molecular biology, tracing reagent and visiualization technology enter the study field of MEOR, and become important took of mechanism study of MEOR. Some correlative catabolism theory of petroleum hydrocarbon and mathematics modei also obtain a series of achievements. The appli-cation of MEOR develops gradually from single well treatment to a whole block of oilfield, and acquire considerable benefit.

生物技術(shù)特別是微生物采油技術(shù),已經(jīng)引起了石油工程技術(shù)人員的空前關(guān)注,目前在國內(nèi)外開展的微生物采油先導性礦場試驗已初見成效,較為典型的當數(shù)美國和俄羅斯,我國的吉林、勝利等油田也進行了礦場試驗,增油效果令人鼓舞。同時,隨著科學技術(shù)的發(fā)展,一些先進的微生物學研究工具已經(jīng)進入微生物采油技術(shù)的研究領(lǐng)域,如分子生物學、示蹤劑及可視化技術(shù)等已經(jīng)成為微生物采油機理研究的重要工具。相關(guān)的石油烴降解理論以及建立數(shù)學模型等也取得了系列成果。微生物采油的應用也從過去的單井處理逐漸向整個區(qū)塊或油田發(fā)展,并見到了明顯效果。

Analyzing bacterial communities in reservoir formation water is necessary in research and development of MEOR methods and technologies by both exogenous and indigenous microorganisms. Seven classes of bacteria commonly existed in oilfield water, beneficial or detrimental to MEOR, are presented. The population of beneficial bacteria (hydrocarbon degrading, denitrifying, and methane producing bacteria) and detrimental bacteria (sulfate reducing, iron, sulfur, and total growth bacteria) in six water samples (of...

Analyzing bacterial communities in reservoir formation water is necessary in research and development of MEOR methods and technologies by both exogenous and indigenous microorganisms. Seven classes of bacteria commonly existed in oilfield water, beneficial or detrimental to MEOR, are presented. The population of beneficial bacteria (hydrocarbon degrading, denitrifying, and methane producing bacteria) and detrimental bacteria (sulfate reducing, iron, sulfur, and total growth bacteria) in six water samples (of recycled produced water for flooding reservoir at block S12 in Shengli and of produced waters from five oil wells of the reservoir) is determined by most probable method and pour plate method and their most probable maximal population is ascertained. It is shown that the formation and produced waters of reservoir at block S12 are comparatively rich in bacterial species, the beneficial and the detrimental bacteria above mentioned are detected in quantitites different for bacterial species and for water samples and their population is generally rather low. It is stated that the oil recovery of the reservoir at block S12 can be enhanced by injecting proper nutrients, activating selectively the stratal microorganisms beneficial to MEOR and inhibiting the detrimental ones in the reservoir formations.

油田水中細菌群落分析,對于外源性和內(nèi)源性微生物采油技術(shù)的研究和開發(fā)都是必要的。介紹了油田水中常見的7類細菌對微生物采油的有益和有害作用。采用三管平行MPN或絕跡稀釋法和澆注平板法對勝利油田S12塊回注污水和5口油井產(chǎn)出水中有益菌(石油烴降解菌HDB,脫氮菌DNB,產(chǎn)甲烷菌MPB)和有害菌(硫酸鹽還原菌SRB,鐵細菌IB,硫細菌SB,腐生菌TGB)群落進行了計數(shù)分析,求得了最大可能含菌量,結(jié)果表明勝利S12塊油藏內(nèi)源微生物群落較豐富,3種有益菌和4種有害菌普遍存在,在各水樣中各類菌的含量有所不同,含量總體較低。認為S12塊油藏可以注入合適的營養(yǎng)物質(zhì),選擇性地激活微生物采油有益菌,抑制有害菌,提高油藏采收率。表2參21。

  本文關(guān)鍵詞：高效石油烴降解菌群的構(gòu)建及其在生物修復中的強化作用研究，由筆耕文化傳播整理發(fā)布。