本文選題：蘇丹黑B　切入點(diǎn)：油脂含量　出處：《中國(guó)油脂》2016年11期 　論文類(lèi)型：期刊論文

【摘要】：以斯氏油脂酵母為研究對(duì)象,探討了利用蘇丹黑B染色定量測(cè)定其胞內(nèi)油脂含量的可行性,對(duì)蘇丹黑B染色法測(cè)定胞內(nèi)油脂含量的條件進(jìn)行了優(yōu)化。結(jié)果表明:斯氏油脂酵母經(jīng)蘇丹黑B染色處理后在600 nm處有最大吸收峰;在沸水浴中加熱6 min和不添加鹽酸的情況下,斯氏油脂酵母蘇丹黑B染色效果最佳;建立了斯氏油脂酵母胞內(nèi)油脂含量與吸光值A(chǔ)600的線性回歸方程,線性關(guān)系顯著(R2=0.992 9)。該方法與酸熱-有機(jī)溶劑法測(cè)定結(jié)果相對(duì)誤差在允許范圍內(nèi),可用于快速測(cè)定斯氏油脂酵母胞內(nèi)油脂含量。
[Abstract]:In order to STEVENSI oleaginous yeast as the research object, discusses the feasibility of using Sultan black B determination of fat content of its intracellular staining, intracellular determination of fat content of Sultan black B staining conditions were optimized. The results show that the STEVENSI oleaginous yeast by Sultan black B staining after treatment at 600 nm with maximum absorption peak; heating in a boiling water bath for 6 min and without adding hydrochloric acid under the condition of STEINERNEMATID oleaginous yeast Sultan black B staining effect is best established; oil content STEVENSI oil yeast cells and the light absorption value of A600 linear regression equation, a significant linear relationship (R2=0.992 9). The measurement results and the heat - acid organic solvent method, the relative error is within the allowable range, can be used for the rapid determination of oil content in STEVENSI oleaginous yeast intracellular.

【作者單位】： 唐山學(xué)院環(huán)境與化學(xué)工程系;
【基金】：河北省教育廳項(xiàng)目(Z2015174) 唐山市科技計(jì)劃項(xiàng)目(15130260a)
【分類(lèi)號(hào)】：TE667;TQ920.1
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本文編號(hào)：1633241