為了獲得高效解有機(jī)磷細(xì)菌,從油茶植株根際土壤中分離到21株解有機(jī)磷細(xì)菌,采用透明圈法進(jìn)行復(fù)篩,僅有4株細(xì)菌能在有機(jī)磷培養(yǎng)基上形成明顯的透明圈。4株細(xì)菌的D/d值在1.62～2.71之間,其中菌株Y6的D/d值最大,為2.71。發(fā)酵上清液有效磷含量為8.50～14.79 mg/L,較CK增加7.88～14.17 mg/L,其中菌株Y6發(fā)酵上清液有效磷含量最多,為14.79 mg/L,較CK增加14.17 mg/L。根據(jù)菌株Y6的菌落形態(tài)特征和生理生化特征及16S rDNA序列分析結(jié)果,初步確定菌株Y6為假單胞菌屬。該菌株有利于改善油茶磷素供應(yīng),促進(jìn)油茶生長,在生物有機(jī)肥研制中具有較大潛力。

【文章頁數(shù)】：7 頁

【文章目錄】：
1. Introduction
2. Materials and Methods
    2.1. Test material
        2.1.1. Sample collection
        2.1.2. Culture medium
    2.2. Test method
        2.2.1. Isolation and purification of organophosphorus solubilizing bacteria
        2.2.2. Rescreening of organophosphorus solubilizing bacteria
        2.2.3. Analysis of phosphorus solubilizing ability
        2.2.4. Identification of organophosphorus solubilizing bacteria
        2.2.5. The relationship between the content of available phosphorus and pH value in the fermentation broth of organophosphorus solubilizing bacteria
        2.2.6. Determination of the growth curve of organophosphorus solubilizing bacteria
        2.2.7. Data processing
3. Results and Analysis
    3.1. Isolation and purification of organoph-osphorus solubilizing bacteria
    3.2. Rescreening of organophosphorus solubilizing bacteria
    3.3. Analysis of the ability of organophosphorus solubilizing bacteria to solubilize phosphorus
    3.4. Identification of organophosphorus solub-ilizing bacteria
        3.4.1. Morphological identification of strains
        3.4.2. Physiological and biochemical characteristics of strains
        3.4.3.16S rDNA sequence analysis of the strain
    3.5. The relationship between pH value and available phosphorus content of fermentation liquid of strain Y6
    3.6. Growth curve of organophosphorus solub-ilizing bacteria Y6
4. Conclusion and Discussion



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