土壤中植物大量營養(yǎng)素（氮、磷、鉀）的有效性是影響作物產(chǎn)量的主要因素之一。這些礦物元素的有效性和形式因土壤類型的不同而有很大的差異。研究表明,通過為植物提供適當?shù)奈⑸锕采w,可以優(yōu)化土壤營養(yǎng)對植物的有效補充。此外,土壤酵母能夠溶解不可利用的磷和鐵,并產(chǎn)生植物生長激素,這些激素可幫助植物生長和維持細菌活性。本研究旨在探討共接種植物促生長細菌（PGPB）—慢生大豆根瘤菌（Bradyrhizobium japonicum）和植物促生長真菌—土壤酵母（Saccharomyces cerevisiae和Saccharomyces exiguus）對大豆（Glycine maxL.Merr）生長和產(chǎn)量的影響,特別是土壤酵母如何影響細菌的空氣氮固定率和結(jié)瘤率。本研究以臺灣292大豆品種為試驗對象,采用完全隨機實驗設計:接種慢生大豆根瘤菌B.japonicum(T1;接種土壤酵母S.cerevisiae(T2;接種S.exiguus（T3）;共接種土壤酵母S.cerevisiae和B.japonicum（T4）;共接種S.exiguous及B.japonicum（T5）以及對照（T6）,每個處理重復4... 

【文章來源】：西南科技大學四川省

【文章頁數(shù)】：50 頁

【學位級別】：碩士

【文章目錄】：
摘要
ABSTRACT
Chapter 1
    1 Background
        1.1 Soybean production and demand
        1.2 Fertilization problems in soybean production
        1.3 Importance of microbes in crop production
        1.4 Research advancements in bio-fertilizer use in crop production
        1.5 Aims and objectives of the study
Chapter 2
    2 Materials and methods
        2.1 Plant material
        2.2 Microbial strains used
        2.3 Experimental design
        2.4 Experimental site location
        2.5 Green house experiment
        2.6 Soil characterisation
            2.6.1 Soil physiochemical parameters
            2.6.2 Soil p H determination
            2.6.3 Soil elemental analysis
        2.7 Inoculum preparation and seed treatment
        2.8 Data collection
            2.8.1 Plant sampling
                2.8.1.1 Soybean germination test
                2.8.1.2 Root elongation bioassay
                2.8.1.3 Nodulation
                2.8.1.4 Soil microbiological analysis
                2.8.1.5 Shoots for collection of plant sap (root bleeding) for determination of atmospheric nitrogen (N2) fixation, dry matter and determination of nitrogen (N) and phosphorus (P) in soybean leaves
                2.8.1.6 Determination of chlorophyll content
                2.8.1.7 Seeds for determination of the nutritional value of harvested seed
                2.8.1.8 Grain yield
                2.8.1.9 Correlation analysis among traits
        2.9 Data analysis
CHAPTER 3
    3 Results
        3.1 Soybean initial growth and development
        3.2 Soybean nodulation
        3.3 Atmospheric nitrogen fixation
        3.4 Yield parameters
Chapter 4
    4 Discussion
        4.1 Soybean initial growth and development
            4.1.1 Seedling shoot analysis
        4.2 Soybean nodulation analysis
            4.2.1 Nodule number and nodule dry weight
        4.3 Chlorophyll content (SPAD Values) leaf area and percentage of total plantnitrogen derived from atmospheric fixation (%Ndfa)
        4.4 Microbial count
        4.5 Yield parameters
Chapter 5
    5 Conclusion
Chapter 6
    6 Recommendations
Chapter 7
    7 Acknowledgements
List of References



本文編號：3175195