【摘要】：【目的】研究不同條件下復(fù)合菌系的秸稈分解能力和酶活性,評價(jià)該復(fù)合菌系的功能穩(wěn)定性及適應(yīng)性,為進(jìn)一步完善該復(fù)合菌系的篩選技術(shù)及其應(yīng)用提供理論依據(jù)�！痉椒ā恳詮�(fù)合菌系GF-20為研究對象,在不同溫度和pH值條件下連續(xù)繼代培養(yǎng),測定所得復(fù)合菌系的濾紙分解特性、玉米秸稈降解率、發(fā)酵液pH值、纖維素酶和半纖維素酶活性,以評價(jià)復(fù)合菌系的功能穩(wěn)定性�！窘Y(jié)果】在溫度4~30℃、pH 6.0~9.0時(shí),經(jīng)過15代的繼代培養(yǎng),復(fù)合菌系GF-20在培養(yǎng)的108~140h可分解濾紙纖維素,玉米秸稈降解率保持在30%以上,發(fā)酵液pH值隨發(fā)酵時(shí)間的延長趨近于中性,pH調(diào)節(jié)能力良好,纖維素酶活性為1.22~2.34 U/mL,半纖維素酶活性為12.82~14.23U/mL。【結(jié)論】復(fù)合菌系GF-20可在較大的溫度和pH范圍內(nèi)保持較高的纖維素和半纖維素酶活性,能發(fā)揮秸稈降解作用,具有良好的開發(fā)潛力和應(yīng)用前景。
[Abstract]:[objective] to study the straw decomposition ability and enzyme activity of the compound strain under different conditions, to evaluate the functional stability and adaptability of the compound strain, and to provide theoretical basis for further perfecting the screening technology and application of the compound strain. [methods] taking the compound strain GF-20 as the research object, the filter paper decomposition characteristics and the degradation rate of corn straw were determined under different temperature and pH conditions. The pH value, cellulase and hemicellulase activity of fermentation broth were used to evaluate the functional stability of the compound strain. [results] when the temperature was 4 鈮，

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