【摘要】：為探討解磷微生物菌肥對香蕉土壤根際微生態(tài)的影響,利用T-RFLP(terminal restriction fragment length polymorphism)技術分析土壤均勻度指數(shù)、香農指數(shù)和優(yōu)勢種群等分析微生物多樣性,研究高效解磷菌劑與化學磷肥的不同組合施用方式對香蕉植株的生長情況和根際土壤微生物物量和群落結構的影響。結果顯示,與對照CK相比,T處理組香蕉苗較CK組生長健壯,地上部分鮮重平均值為42.2 cm,明顯比CK高出35.2%。接M-3-01解磷細菌處理組T1、T3生長勢最為顯著(P0.05),其中,T3株高和莖圍分別是CK的1.26、1.16倍。T-RFLP技術分析微生物多樣性表明,T1、T3多樣性指數(shù)和均勻度指數(shù)指標分別為2.12/2.11和0.93/0.88,與CCK差異性不明顯,但略微高于CK。同時,分析土壤優(yōu)勢種群,處理組T1、T3處理組土壤優(yōu)勢微生物種群數(shù)分別為12和14,數(shù)目基本與處理前土壤CCK的一致,而T2略低于CK。擬柱孢藻(Cylindrospermopsis)和北里孢菌(Kitasatospora)只存在于CK和施用解磷菌劑T1、T3處理土壤中。土壤種植香蕉苗后,不同施用方式對土壤微生物群落豐富度和多樣性的穩(wěn)定具有積極影響。因此,施用高效解磷菌劑對維持土壤微生物群落豐富度和均勻度效果較好。
[Abstract]:In order to study the effect of phosphorus-hydrolyzing microbial fertilizer on rhizosphere microecology of banana soil, soil evenness index, Shannon index and dominant population were analyzed by T-RFLP (terminal restriction fragment length polymorphism) technique. The effects of different combination of high efficiency phosphorus remover and chemical phosphate fertilizer on the growth of banana plants and the microbial biomass and community structure of rhizosphere soil were studied. The results showed that compared with the control CK, the growth of banana seedlings in T treatment group was stronger than that in CK group, and the average fresh weight of aerial parts in T treatment group was 42.2 cm, higher than that in CK group (35.2%). The growth potential of T3 was the most significant (P 0.05) in the treatment group T _ 1 treated with M 鈮，

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