【摘要】：為研究不同植被下土壤微生物群落結(jié)構(gòu)多樣性的差異,采用磷脂脂肪酸(PLFA)生物標(biāo)記法,對(duì)山西吉縣黃土丘陵區(qū)典型人工林刺槐、油松以及荒草地的土壤微生物群落結(jié)構(gòu)多樣性進(jìn)行分析研究。結(jié)果表明:不同植被下土壤微生物PLFA標(biāo)記含量存在顯著差異,刺槐人工林的土壤微生物PLFA總量最大,細(xì)菌、真菌、放線菌的PLFA含量均大于油松人工林和荒草地;細(xì)菌PLFA含量在3種樣地中的差異顯著;2種人工林的真菌PLFA含量接近,而與荒草地差異顯著,與荒草地相比,人工林的種植可明顯提高土壤中真菌的比例;革蘭氏陽(yáng)性菌與革蘭氏陰性菌呈現(xiàn)相同的變化趨勢(shì),油松人工林與荒草地2種菌群的PLFA含量接近,而與刺槐人工林差異顯著。通過(guò)相關(guān)性分析發(fā)現(xiàn),不同植被下土壤細(xì)菌、真菌、革蘭氏陽(yáng)性菌與革蘭氏陰性菌各總PLFA與土壤養(yǎng)分因子之間密切相關(guān);不同植被下土壤微生物群落多樣性差異顯著,刺槐人工林更為豐富,且各個(gè)菌群分布更為均勻,油松人工林土壤的微生物群落多樣性及菌群分布均勻度與刺槐人工林土壤較為接近,荒草地土壤微生物群落多樣性和均勻程度較2種人工林明顯偏低。因此從微生物群落結(jié)構(gòu)多樣性的角度看,人工林的種植能夠明顯改善微生物群落結(jié)構(gòu),改善土壤質(zhì)量;刺槐人工林是黃土丘陵區(qū)人工植被恢復(fù)的較好選擇。
[Abstract]:In order to study the diversity of soil microbial community structure under different vegetation, phospholipid fatty acid (PLFA) biomarker method was used to study Robinia pseudoacacia (Robinia pseudoacacia) in loess hilly region of Jixian County, Shanxi Province. The diversity of soil microbial community structure in Pinus tabulaeformis and wasteland was analyzed and studied. The results showed that there were significant differences in PLFA marker content of soil microorganisms under different vegetation. The total amount of soil microbial PLFA in Robinia pseudoacacia plantation was the largest, and the PLFA content of bacteria, fungi and actinomycetes was higher than that of Pinus tabulaeformis plantation and wasteland. The content of bacterial PLFA in the three plots was significantly different, and the content of fungal PLFA in the two plantations was close to that in the wasteland, but significantly different from that in the wasteland. Compared with the wasteland, the planting of the plantation could significantly increase the proportion of fungi in the soil. Gram-positive bacteria and Gram-negative bacteria showed the same change trend. The PLFA content of Pinus tabulaeformis plantation and wasteland was close to that of Robinia pseudoacacia plantation, but significantly different from that of Robinia pseudoacacia plantation. Through correlation analysis, it was found that the total PLFA of soil bacteria, fungi, Gram-positive bacteria and Gram-negative bacteria under different vegetation were closely related to soil nutrient factors. There were significant differences in soil microbial community diversity under different vegetation, Robinia pseudoacacia plantation was more abundant, and the distribution of each flora was more uniform. The soil microbial community diversity and flora distribution evenness of Pinus tabulaeformis plantation were close to those of Robinia pseudoacacia plantation, and the diversity and uniformity of soil microbial community of wasteland grassland were significantly lower than those of the two plantations. Therefore, from the point of view of microbial community structure diversity, plantation can obviously improve microbial community structure and soil quality, and Robinia pseudoacacia plantation is a good choice for artificial vegetation restoration in loess hilly region.
【作者單位】： 北京林業(yè)大學(xué)水土保持與荒漠化防治教育部重點(diǎn)實(shí)驗(yàn)室 山西吉縣國(guó)家森林生態(tài)系統(tǒng)野外觀測(cè)研究站;
【基金】：“十二五”國(guó)家科技支撐計(jì)劃項(xiàng)目(2015BAD07B02) 國(guó)家生態(tài)系統(tǒng)觀測(cè)研究網(wǎng)絡(luò)運(yùn)行服務(wù)項(xiàng)目(2014—2015)
【分類號(hào)】：S714.3

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