【摘要】：預處理是生物質(zhì)能源產(chǎn)品制備中最昂貴、最關鍵的環(huán)節(jié),生物預處理因環(huán)境友好和能耗低而受到廣泛關注。為獲得較好的生物預處理菌株,以脫膠效率為出發(fā)點篩選菌株,從鳳眼蓮(Eichhornia crassipes)生長環(huán)境介質(zhì)中分離菌株,通過富集培養(yǎng)、剛果紅平板染色法初篩,獲得一株對鳳眼蓮脫膠效果較好的A_1菌株。在最佳條件下A_1菌株分泌的果膠酶活性、木聚糖酶活性和纖維素酶活性分別為3 925.00、8 331.67和4 883.62 nmol·s~(-1)·mL~(-1);A_1菌株對鳳眼蓮的1次脫膠減重率為33.37%,3次連續(xù)脫膠的累計減重率為49.26%。通過16S rRNA序列鑒定,構建系統(tǒng)發(fā)育樹分析,該菌株為解淀粉芽孢桿菌(Bacillus amyloliquefaciens)。
[Abstract]:Pretreatment is the most expensive and critical link in the preparation of biomass energy products. Biological pretreatment has been widely concerned because of its friendly environment and low energy consumption. In order to obtain a better biological pretreatment strain, the strains were screened from the growth environment of Eichhornia crassipes (Eichhornia crassipes) according to the degumming efficiency. The strains were isolated from the growth environment of Eichhornia crassipes. The strains were screened by enrichment culture and Congo red plate staining. A strain A 鈮，

本文編號：2482365