【摘要】：以甜瓜連作土壤為研究對象,采用高效液相色譜技術(shù),分析了不同連作年限甜瓜土壤中酚酸物質(zhì)的種類及其含量變化,利用生防木霉菌降解特性,降解土壤中酚酸類物質(zhì),為緩解甜瓜連作障礙提供參考依據(jù)。結(jié)果表明:連作甜瓜土壤中對羥基苯甲酸、香草酸、丁香酸、香蘭素、香豆酸、阿魏酸、苯甲酸隨著連作年限的增加而增加,連作3年后7種酚酸的總量達245.39mg·kg~(-1)干土,顯著高于未連作土壤;采用孢子菌懸液、PD菌懸液和麥麩發(fā)酵物3種不同的接種方式,測定木霉菌T23對甜瓜根際土壤中酚酸類物質(zhì)的降解作用,表明木霉菌T23能夠有效降解土壤中積累的酚酸物質(zhì),其中麥麩發(fā)酵物降解率可達到25%以上,降解效果最為顯著。
[Abstract]:The phenolic acids in muskmelon soil under continuous cropping were analyzed by high performance liquid chromatography (HPLC). The phenolic acids were degraded by Trichoderma biocontrol. To provide reference basis for alleviating the obstacle of continuous cropping of muskmelon. The results showed that p-hydroxybenzoic acid, vanillic acid, eugenic acid, vanillin, coumaric acid, ferulic acid and benzoic acid increased with the increase of continuous cropping time. After 3 years of continuous cropping, the total amount of 7 phenolic acids reached to 245.39mg kg~ (-1) dry soil, which was significantly higher than that of non-continuous cropping soil. The degradation of phenolic acids in muskmelon rhizosphere soil by Trichoderma T23 was determined by three different inoculation methods: spore suspension, PD suspension and wheat bran fermentation. The results showed that Trichoderma T23 could effectively degrade the phenolic acid accumulated in the soil. The degradation rate of wheat bran fermentation was more than 25%, and the degradation effect was the most remarkable.
【作者單位】： 沈陽農(nóng)業(yè)大學植物保護學院;中國科學院沈陽應(yīng)用生態(tài)研究所;
【基金】：公益性行業(yè)(農(nóng)業(yè))科研專項經(jīng)費資助項目(201503110-04)
【分類號】：S652;S153

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