【摘要】：對(duì)兩室根箱試驗(yàn)裝置中一分室(供體室)定植的枳Poncirus trifoliata實(shí)生苗接種叢枝菌根(AM)真菌Paraglomus occultum,另一分室(受體室)不作任何處理。定植17周后,將潰瘍病病菌Xanthomonas axonopodis(Xac)接種于供體植株,研究不同處理受體植株間的信號(hào)物質(zhì)響應(yīng)差異,揭示菌絲橋的功能。與不接種處理相比,單接種AM真菌、預(yù)先接種AM真菌后再接種Xac均顯著提高了供體根系一氧化氮(NO)含量,單接種AM真菌只顯著提高了受體根系NO含量;單接種Xac顯著降低了供體根系脫落酸(ABA)含量,而單接種AM真菌、雙接種(AM真菌+Xac)均增加了供體根系A(chǔ)BA含量。雙接種處理顯著提高了受體ABA含量;與不接種對(duì)照相比,接種處理均降低了供體根系玉米素核苷(ZR)含量,同時(shí)單接種AM真菌、雙接種處理也降低了受體根系ZR含量;AM真菌顯著降低了供體和受體根系茉莉酸(JA)含量,但雙接種卻顯著提高了供體和受體根系JA含量;與不接種對(duì)照相比,單接種AM真菌或Xac、雙接種處理均顯著下調(diào)供體根系JA合成基因(PtLOX、PtAOS和PtAOC)的表達(dá),單接種Xac顯著下調(diào)了受體根系PtLOX和Pt AOC的表達(dá),單接種AM真菌顯著下調(diào)了受體根系PtAOC的表達(dá),而雙接種顯著上調(diào)了受體根系PtAOS基因的表達(dá)。表明菌根真菌的菌絲橋傳導(dǎo)JA并誘導(dǎo)受體植株中NO、ABA和ZR含量改變以抵御潰瘍病菌危害。
[Abstract]:In the two-compartment root box test system, the one-compartment (donor room) seeding of trifoliate orange (Trifoliate trifoliate) Poncirus trifoliata seedlings with arbuscular mycorrhizal fungi (AM) and Paraglomus occultum, (receptor chamber) was not treated. After 17 weeks of colonization, Xanthomonas axonopodis (Xac) was inoculated into donor plants to study the difference of signal substance response among the recipient plants, and to reveal the function of hyphal bridge. Compared with no inoculation, inoculation of AM fungi alone, inoculation of AM fungi before inoculation with Xac significantly increased the content of nitric oxide (NO) in donor roots, and AM fungi inoculated only increased NO content in recipient roots. The (ABA) content of abscisic acid in donor roots was significantly decreased by single inoculation of Xac, while ABA content in donor roots was increased by Xac inoculation with AM fungi. Compared with the control group, the (ZR) content of zeatin nucleoside in donor root system was decreased by double inoculation treatment, and the ZR content in recipient root system was also decreased by double inoculation treatment and AM fungi inoculation alone. AM fungi significantly decreased the content of (JA) in donor and receptor roots, but increased the JA content of donor and receptor roots by double inoculation. The expression of JA synthase genes (PtLOX,PtAOS and PtAOC) in donor roots was significantly down-regulated by single inoculation of AM fungi or double inoculation of Xac, and the expression of PtLOX and Pt AOC in recipient roots was significantly down-regulated by single inoculation of Xac. Single inoculation of AM fungi significantly down-regulated the expression of PtAOC in receptor root system, while double inoculation significantly up-regulated the expression of PtAOS gene in receptor root system. The results indicated that mycelial bridge of mycorrhizal fungi transduces JA and induces changes of NO,ABA and ZR contents in the recipient plants in order to resist the harm of ulcers.
【作者單位】： 長江大學(xué)園藝園林學(xué)院長江大學(xué)根系生物學(xué)研究所;
【基金】：湖北省高等學(xué)校優(yōu)秀中青年科技創(chuàng)新團(tuán)隊(duì)計(jì)劃項(xiàng)目(T201604)~~
【分類號(hào)】：S436.66;S476.1
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本文編號(hào)：2372828