【摘要】：[目的]從長期被氟樂靈污染的土壤中篩選出能以氟樂靈為唯一碳氮源生長的細菌,并對其降解特性進行研究。[方法]以連作15年以上棉田土壤為材料,采用直接稀釋涂布法分離得到1株能夠降解氟樂靈的細菌D8,通過形態(tài)特征、生理生化特性和16S r DNA序列分析,對菌株D8進行鑒定;通過單因素試驗研究降解特性。[結果]鑒定菌株D8為蠟樣芽孢桿菌(Bacillus cereus),在氟樂靈質量濃度為50 mg/L、接種量為4%、外加氮源酵母浸粉0.1%、溫度37℃、p H值為5.0的條件下,接種3 d后對氟樂靈降解率達到59.89%以上。[結論]該研究為氟樂靈的生物降解和土壤修復提供理論依據(jù)。
[Abstract]:[objective] to screen the bacteria that can grow with florazone as the sole carbon and nitrogen source from the soil contaminated with florramine for a long time, and to study the degradation characteristics of the bacteria. [methods] A strain D8 was isolated from cotton field soil for more than 15 years by direct dilution coating method. The strain D8 was identified by morphological, physiological and biochemical characteristics and 16s r DNA sequence analysis. The degradation characteristics were studied by single factor test. [results] the strain D8 was identified as Bacillus cereus (Bacillus cereus),. Under the conditions of 50 mg/L, inoculation with 50 mg/L, of florazone, 0.1% yeast extract with nitrogen source and 5.0, p H at 37 鈩，

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