土壤病毒生態(tài)學(xué)研究方法
發(fā)布時(shí)間:2018-11-23 12:30
【摘要】:病毒是地球上最豐富的生物實(shí)體,每克土壤中可包含數(shù)以億計(jì)的病毒,它不僅影響土壤中其它微生物的群落組成、土壤元素的生物地球化學(xué)循環(huán),還會(huì)影響土壤微生物的物種進(jìn)化,甚至影響植物、動(dòng)物和人體健康。目前人們對(duì)土壤中病毒的種類及豐度、分布特征以及功能引起的生態(tài)環(huán)境效應(yīng)還知之甚少。在概述病毒生態(tài)學(xué)研究方法的基礎(chǔ)上,對(duì)土壤病毒的提取、純化、定量及分子生態(tài)學(xué)方法等基本流程進(jìn)行了比較分析,以期建立一套快速簡(jiǎn)便、高效穩(wěn)定的適用于土壤病毒研究的方法,并用于研究土壤病毒的多樣性及分布特征,探討病毒在環(huán)境中的生存和傳播機(jī)制,為土壤病毒的防控及開發(fā)利用提供支撐。
[Abstract]:Viruses are the most abundant biological entities on the planet, containing hundreds of millions of viruses per gram of soil, affecting not only the community composition of other microbes in the soil, but also the biogeochemical cycle of soil elements. It also affects the evolution of soil microbes, and even plants, animals and human health. At present, little is known about the ecological and environmental effects caused by the species, abundance, distribution and function of viruses in soil. On the basis of summarizing the research methods of virus ecology, the extraction, purification, quantification and molecular ecology methods of soil viruses were compared and analyzed in order to establish a set of rapid and simple methods. It can be used to study the diversity and distribution of soil viruses, to study the survival and transmission mechanism of viruses in the environment, and to provide support for the prevention, control, development and utilization of soil viruses.
【作者單位】: 中國(guó)科學(xué)院生態(tài)環(huán)境研究中心城市與區(qū)域生態(tài)國(guó)家重點(diǎn)實(shí)驗(yàn)室;中國(guó)科學(xué)院大學(xué);
【基金】:國(guó)家自然科學(xué)基金資助項(xiàng)目(41571248,41301265,41230857)
【分類號(hào)】:S154.3
本文編號(hào):2351618
[Abstract]:Viruses are the most abundant biological entities on the planet, containing hundreds of millions of viruses per gram of soil, affecting not only the community composition of other microbes in the soil, but also the biogeochemical cycle of soil elements. It also affects the evolution of soil microbes, and even plants, animals and human health. At present, little is known about the ecological and environmental effects caused by the species, abundance, distribution and function of viruses in soil. On the basis of summarizing the research methods of virus ecology, the extraction, purification, quantification and molecular ecology methods of soil viruses were compared and analyzed in order to establish a set of rapid and simple methods. It can be used to study the diversity and distribution of soil viruses, to study the survival and transmission mechanism of viruses in the environment, and to provide support for the prevention, control, development and utilization of soil viruses.
【作者單位】: 中國(guó)科學(xué)院生態(tài)環(huán)境研究中心城市與區(qū)域生態(tài)國(guó)家重點(diǎn)實(shí)驗(yàn)室;中國(guó)科學(xué)院大學(xué);
【基金】:國(guó)家自然科學(xué)基金資助項(xiàng)目(41571248,41301265,41230857)
【分類號(hào)】:S154.3
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