【摘要】：【目的】擬針對禾谷鐮孢進(jìn)行生防菌的篩選和應(yīng)用研究,以期為玉米莖基腐病生防菌劑的研制奠定基礎(chǔ)。【方法】通過對峙培養(yǎng)法對玉米莖基腐病的主要致病菌禾谷鐮孢進(jìn)行玉米內(nèi)生生防細(xì)菌的篩選,從玉米主栽品種九單48幼苗內(nèi)部獲得一株具有較強(qiáng)抑菌效果的內(nèi)生生防細(xì)菌(簡稱48SJ7-1);基于傳統(tǒng)鑒定、16S rRNA基因序列測定及系統(tǒng)發(fā)育分析,對48SJ7-1進(jìn)行鑒定;并通過盆栽試驗(yàn)測定該生防菌株的防效�！窘Y(jié)果】菌株48SJ7-1經(jīng)鑒定為甲基營養(yǎng)型芽孢桿菌,GenBank登錄號為KU377993,48SJ7-1盆栽防效為68.47%,與對照藥劑2%戊唑醇懸浮種衣劑差異不顯著�！窘Y(jié)論】48SJ7-1對玉米莖基腐病的主要致病菌禾谷鐮孢有較好防效,對玉米生長還有明顯的促進(jìn)作用,而且表觀上無藥害發(fā)生。
[Abstract]:[objective] to study the screening and application of biological control bacteria against Fusarium graminearum, In order to lay a foundation for the preparation of biocontrol agent of maize stem base rot. [methods] screening the bacteria of Fusarium graminearum, the main pathogen of maize stem rot, by means of confrontation culture, An internal bacteriostasis strain (48SJ7-1) with strong bacteriostatic effect was obtained from the seedlings of main maize cultivar Jiudan 48, and 48SJ7-1 was identified based on the sequencing of 16s rRNA gene and phylogenetic analysis. The control effect of the biocontrol strain 48SJ7-1 was determined by pot experiment. [results] the strain 48SJ7-1 was identified as Bacillus spp. The accession number of GenBank was KU377993A48SJ7-1, and the control effect was 68.47%. There was no significant difference between the strain 48SJ7-1 and 2% tebuconazole suspension seed coating agent. [conclusion] 48SJ7-1 has better control effect on Fusarium graminearum, the main pathogen of maize stem base rot. It can also promote the growth of maize, and there is no apparent harm.
【作者單位】： 東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)院黑龍江省高校寒地作物品種改良與生理生態(tài)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家重點(diǎn)研發(fā)計劃項(xiàng)目(No.2017YFD0201803) 科技部重點(diǎn)專項(xiàng)項(xiàng)目(No.2016YFD0300704)~~
【分類號】：S435.131.4;S476.1

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