本文選題：農(nóng)作物秸稈 + 纖維素降解菌��； 參考：《環(huán)境科學(xué)學(xué)報(bào)》2017年10期

【摘要】：采用單一碳源選擇性培養(yǎng)基和纖維素平板水解圈法篩選到一株具有較強(qiáng)纖維素分解能力的菌株Arthrobacter oryzae HW-17.此外,高通量測(cè)序發(fā)現(xiàn),不同馴化條件下微生物群落結(jié)構(gòu)有明顯差異,低溫條件下優(yōu)勢(shì)屬為類芽孢桿菌屬(Paenibacillus)和伯克氏菌屬(Burkholderia).本文同時(shí)對(duì)菌株Arthrobacter oryzae HW-17的微生物特性和纖維素降解特性進(jìn)行了初步研究,結(jié)果發(fā)現(xiàn),KNO_3、30或35℃、pH=7分別為菌株產(chǎn)纖維素酶的最佳氮源、溫度和pH.菌株HW-17的最高纖維素酶活為18.55 U·m L~(-1),且對(duì)磨碎加工處理的纖維素樣品和含雞糞的纖維素混合樣品有更好的降解效果.此外,菌株HW-17產(chǎn)生的纖維素酶在中溫(≤50℃)和偏酸性(pH=5~7)條件下能保持較高的酶活.Cu(Ⅱ)和Pb(Ⅱ)等金屬離子能夠抑制該酶的活性.
[Abstract]:A strain Arthrobacter oryzae HW-17 with strong cellulose decomposition ability was screened by single carbon selective medium and cellulose plate hydrolysis circle method. In addition, high throughput sequencing showed that there were significant differences in microbial community structure under different acclimation conditions, and the dominant species at low temperature were Paenibacillus and Burkholderia. The microbial characteristics and cellulose degradation characteristics of strain Arthrobacter oryzae HW-17 were also studied in this paper. The results showed that the optimum nitrogen source, temperature and pH 7 for cellulase production were 30 鈩，

本文編號(hào)：2084067