本文選題：菲 + 芘��； 參考：《浙江大學(xué)》2017年碩士論文

【摘要】：多環(huán)芳烴(Polycyclic Aromatic Hydrocarbons,PAHs)是一種環(huán)境中廣泛分布的有機(jī)污染物,具有致畸、致癌和致突變等毒性,嚴(yán)重危害著人類健康以及生態(tài)環(huán)境。如何去除環(huán)境中的PAHs引起了全世界的廣泛研究,許多研究表明微生物修復(fù)是高效去除環(huán)境中PAHs的主要手段之一。因此,獲得PAHs高效降解菌以及生物修復(fù)過程中降解菌的修復(fù)效果等對實(shí)際環(huán)境中的PAHs污染的微生物治理具有非常重要的指導(dǎo)意義。本研究從PAHs污染的土壤中篩選分離獲得了一株高效降解菌WY10,并對這株菌進(jìn)行了鑒定;同時探究了菌株WY10降解菲和芘的影響因素,以及菌株WY10在菲和芘污染土壤修復(fù)中的應(yīng)用和修復(fù)過程中PAHs降解基因的豐度變化,得到了以下主要研究結(jié)果:(1)通過形態(tài)學(xué)、生理生化以及16SrRNA、hsp65、recA和r叩oB基因的序列分析等手段進(jìn)行鑒定,判定菌株WY10屬于分枝桿菌屬(Mycobacterium)。(2)菌株WY10可以分別在pH(4.5-8.5)和溫度(20-35 ℃)的范圍內(nèi)高效地降解菲和芘。菲和芘的最佳降解條件分別是:pH 4.5-7.5、溫度30-35 ℃和pH 4.5-7.5、溫度28-35℃。在pH6.5、溫度28℃條件下培養(yǎng)72h,菲(100mg·L-1)和芘(50 mg·L-1)的降解率分別高達(dá)100.0%和92.3%。除了培養(yǎng)24h之前葡萄糖和麥芽糖的添加會促進(jìn)菌株WY10對芘的降解外,果糖、葡萄糖和麥芽糖添加濃度為100mgL-1時,對菌株WY10降解菲和芘都沒有顯著的影響。(3)菌株WY10對土壤中的菲和芘也有非常高的降解效果,接種WY10的土壤培養(yǎng)35天后,菲和芘的濃度分別從39.8 mg·kg-1和18.2 mg·kg-1快速降解到后3.0mg·kg-1和1.1mg·kg-1;土壤中的土著微生物也能降解土壤中的菲和芘,降解后期對照處理土壤中菲和芘的殘留濃度分別為5.0 mg·kg-1和2.9 mg·kg-1。(4)土壤中Mycobacterium 16S rRNA、RHDα-GP、nidA、nidB基因豐度都隨著菲和芘的降解而逐漸增加,同時土壤中菲和芘的殘留濃度與Mycobacterium 16S rRNA、RHDα-GP、nid4、nidB基因豐度呈現(xiàn)非常高的負(fù)相關(guān)性,說明土壤中分枝桿菌以及攜帶有RHDα-GP、nidA和nindB基因的微生物對土壤中菲和芘的降解起到非常重要的作用。
[Abstract]:Polycyclic Aromatic hydrocarbons (PAHs) are widely distributed organic pollutants in the environment, with teratogenic, carcinogenic and mutagenic toxicity, which seriously harm human health and ecological environment. How to remove PAHs from the environment has caused extensive research all over the world. Many studies have shown that microbial remediation is one of the main methods to efficiently remove PAHs from the environment. Therefore, it is very important to obtain the efficient degradation bacteria of PAHs and the remediation effect of biodegradable bacteria in the process of bioremediation for the treatment of PAHs pollution in the actual environment. In this study, a strain WY10 was isolated from PAHs contaminated soil and identified, and the factors influencing the degradation of phenanthrene and pyrene by strain WY10 were investigated. The application of strain WY10 in phytoremediation of phenanthrene and pyrene contaminated soil and the changes in the abundance of PAHs degradation genes during remediation were obtained. The main results were as follows: (1) Morphology, Physiological and biochemical methods and sequence analysis of 16s rRNA hsp65recA and r koroB genes were used to identify the strain WY10, which belongs to Mycobacterium). (2 strain, which can efficiently degrade phenanthrene and pyrene in the range of pH (4.5-8.5) and temperature (20-35 鈩，

本文編號：2059535