本文選題：棉鈴蟲 + 絲氨酸蛋白酶抑制劑基因　； 參考：《基因組學與應用生物學》2017年04期

【摘要】：本研究對棉鈴蟲絲氨酸蛋白酶抑制劑(Helicoverpa armigera serine protease inhibitor,Haspi)基因的基本特征、分布情況及功能進行了初步探究。通過與已報道的家蠶絲氨酸蛋白酶抑制劑基因同源比對,利用RT-PCR技術成功克隆到3條與家蠶絲氨酸蛋白酶抑制劑基因相似性高的Haspi基因,分別命名為:Haspi-5、Haspi-6和Haspi-10。實時定量PCR結果表明3個基因在棉鈴蟲各組織部位均有轉錄,但在不同組織部位處的表達水平差異顯著。Haspi-5基因在表皮中的表達水平最高;Haspi-6基因在頭部的轉錄水平遠遠高于其他組織部位;Haspi-10基因在前腸中表達水平最高,中腸的轉錄水平也相比其他組織較高。構建表達Haspi-5、Haspi-6基因的ds RNA的載體,用表達ds RNA的HT115菌液喂食,其中Haspi-5基因在棉鈴蟲的轉錄水平明顯下降,約降低了6.5倍,而Haspi-6基因轉錄水平略有下降但并不明顯,約降低了1.2倍,但是沒有觀察到明顯的表型變化。上述研究為Haspi基因的功能及棉鈴蟲防治研究提供科學依據(jù)。
[Abstract]:In this study, the basic characteristics, distribution and function of Helicoverpa armigera serine protease inhibitor (Haspi) gene were preliminarily explored. By homologous comparison with the reported homologous sericulate protease inhibitor gene, 3 sericulture eggs were successfully cloned by RT-PCR technology. The Haspi gene with high similarity of white enzyme inhibitor gene was named as: Haspi-5, Haspi-6 and Haspi-10. real-time quantitative PCR results showed that 3 genes were transcribed in the tissues of Helicoverpa armigera, but the expression level of the.Haspi-5 gene was the highest in the epidermis of the.Haspi-5 gene at different tissues, and the Haspi-6 gene was transferred to the head. The level of the Haspi-10 gene was highest in the foregut and the highest in the foregut, and the transcriptional level of the midgut was higher than that of other tissues. The carrier of the DS RNA expressing the Haspi-5 and Haspi-6 gene was fed with the HT115 bacteria expressing the DS RNA, and the Haspi-5 gene in the transcriptional level of the Helicoverpa armigera was obviously reduced by about 6.5 times. The transcriptional level of Haspi-6 gene declined slightly but was not obvious, which was about 1.2 times lower, but no obvious phenotypic changes were observed. The study provided a scientific basis for the function of Haspi gene and the prevention and control of Helicoverpa armigera.
【作者單位】： 河北大學生命科學學院;
【基金】：河北省教育廳重點項目(ZD2016141) 河北省自然科學基金(C2014201056)共同資助
【分類號】：S433

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本文編號：2055181