本文選題：田七 + 根際土壤��； 參考：《廣西大學(xué)》2015年碩士論文

【摘要】：田七是在廣西百色市栽培的五加科人參屬植物三七[Panax notoginseng (Burk.) F.H.Chen],是一種名貴的中藥材。為了探討田七連作障礙與田七根際微生物之間的聯(lián)系,本文以不同的田七根際土壤為實驗材料,對株齡和連續(xù)種植年限不同的田七植株的根際土壤的細(xì)菌、真菌、放線菌數(shù)量變化及其酶活性、pH值等生理特征進(jìn)行了比較研究。研究結(jié)果如下：1、隨著田七植株株齡的增加,田七根際土壤細(xì)菌、真菌和放線菌的數(shù)量均有不同程度的減少,其減少數(shù)量多少依次為：CK(未種過田七土壤)1年植株2年植株3年植株,說明田七植株株齡對根際土壤的微生物有較大影響。2、隨著田七植株株齡的增加,植株根際土壤的過氧化氫酶、脲酶、酸性磷酸酶活性及pH值都降低。酶活性、pH值大小依次為：：CK1年植株2年植株3年植株,說明田七植株株齡大小影響根際土壤的酶活性和土壤pH值。3、連續(xù)種植年限對田七根際細(xì)菌、放線菌數(shù)量有明顯影響。研究表明,田七根際土壤細(xì)菌和放線菌數(shù)量隨著連續(xù)種植年限增加而減少；茬后土壤,即采收田七撂荒后,兩種菌的數(shù)量增加,其大小依次為：CK2(未種過田七土壤)茬后土壤連續(xù)種植2年連續(xù)種植3年。說明種植年限的增加使根際土壤細(xì)菌、放線菌的生長繁殖受到抑制,撂荒后兩種菌數(shù)量回升。4、根際土壤真菌數(shù)量隨著田七種植年限的增加而增加,且與種植年限呈正比；茬后土壤,即采收田七撂荒后的土壤,真菌數(shù)量變少,說明連續(xù)種植年限的增加有利于真菌的生長繁殖,撂荒后可減少真菌數(shù)量。5、連續(xù)種植年限對田七根際土壤酶活性及pH值也有較大影響。根際土壤的過氧化氫酶、脲酶、酸性磷酸酶活性及pH值,隨著連續(xù)種植年限的增加而降低：但采收田七撂荒后的土壤,又出現(xiàn)升高趨勢。酶活性大小、pH值依次為：CK2(未種過田七土壤)茬后土壤連續(xù)種植2年連續(xù)種植3年。說明連續(xù)種植年限抑制了其根際土壤的酶活性和降低土壤pH值,撂荒后可改變這個現(xiàn)象。6、田七根際土壤真菌和細(xì)菌數(shù)量都隨著氣溫升高和降雨量的增加,有逐漸降低的趨勢；然后隨著氣溫的下降和降雨量的減小,細(xì)菌和真菌數(shù)量出現(xiàn)回升。放線菌數(shù)量隨著氣溫升高和降雨量的增加,出現(xiàn)先降低后升高的趨勢；然后隨著氣溫的下降和降雨量的減少,出現(xiàn)先降低后升高的趨勢。說明氣候可以影響微生物群落結(jié)構(gòu)。
[Abstract]:Tianqi is a panax notoginseng of Panax notoginseng Burk. cultivated in Baise, Guangxi. F. H. Chen is a valuable Chinese medicine. In order to study the relationship between continuous cropping obstacle and rhizosphere microorganism, bacteria and fungi in rhizosphere soil with different plant ages and different planting years were used as experimental materials. The changes of actinomycetes quantity and the pH value of enzyme activity were compared. The results are as follows: 1. With the increase of plant age, the number of bacteria, fungi and actinomycetes in the rhizosphere of Tianqi decreased in varying degrees. The order of the decrease was 1 year, 2 years, 3 plants, which indicated that the plant age had a great influence on the microorganism in rhizosphere soil, and with the increase of plant age, The activities of catalase, urease, acid phosphatase and pH of rhizosphere soil decreased. The order of pH value of enzyme activity and pH value was 1: 1, 2 years and 3 years, which indicated that the plant age of Tianqi plant affected the enzyme activity and soil pH value of rhizosphere soil, and the number of actinomycetes and bacteria in rhizosphere was affected obviously by continuous planting years. The results showed that the number of bacteria and actinomycetes in rhizosphere soil of Tianqi decreased with the increase of successive planting years, and the number of two bacteria in soil after stubble, that is, after the harvest was abandoned, increased. The order of its size is: 1: CK2 (no field 7 soil) after stubble, the soil is planted for 2 years and 3 years in succession. The results showed that the growth and reproduction of rhizosphere soil bacteria and actinomycetes were inhibited by the increase of planting years, and the number of two fungi increased by .4. the number of fungi in rhizosphere soil increased with the increase of planting years, and it was proportional to the years of planting. The number of fungi in the soil after stubble, that is, after the harvest field was abandoned, became smaller, which indicated that the increase of continuous planting years was beneficial to the growth and reproduction of fungi. The number of fungi decreased by 0.05 after abandoned, and the enzyme activity and pH value of rhizosphere soil were also affected by continuous planting years. The activities of catalase, urease, acid phosphatase and pH value of rhizosphere soil decreased with the increase of successive planting years. The pH value of enzyme activity was 1: CK2 (no soil planted in Tianqi). The soil was planted continuously for 2 years and 3 years. The results showed that continuous planting years inhibited the enzyme activity of rhizosphere soil and decreased the soil pH value, which could be changed by abandonment. The number of fungi and bacteria in rhizosphere soil of Tianqi decreased gradually with the increase of temperature and rainfall. Then, with the decrease of temperature and rainfall, the number of bacteria and fungi increased. The number of actinomycetes decreased first and then increased with the increase of temperature and rainfall, and then decreased first and then increased with the decrease of temperature and rainfall. It shows that climate can affect the structure of microbial community.
【學(xué)位授予單位】：廣西大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S567.236;S154.3

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