本文選題：懷地黃 + 內(nèi)生細(xì)菌�。� 參考：《江蘇農(nóng)業(yè)科學(xué)》2017年13期

【摘要】：從藥用植物懷地黃的根中分離出32株內(nèi)生細(xì)菌,并選擇大腸桿菌、金黃色葡萄球菌、黑曲霉、地黃斑枯病和地黃根腐病5種菌作為供試菌株,采用點菌法、平板對峙法和菌餅法測定抗菌活性。結(jié)果顯示,懷地黃中分離的32株內(nèi)生菌對5種供試菌具有不同的抑制能力,其中有8株細(xì)菌對供試的大腸桿菌、金黃色葡萄球菌和黑曲霉都有抑制作用,顯示出廣譜的抗菌性;有2株細(xì)菌DH-10和DH-11對選取的5種供試菌都有抑制作用,且抗菌活性較強,具有生防菌開發(fā)潛力。所分離的活性內(nèi)生菌通過16S rRNA基因序列分析,主要分布在芽孢桿菌屬、假單胞菌屬和大腸埃希氏菌屬,其中對地黃斑枯病和根腐病都具有拮抗作用的內(nèi)生菌主要集中在芽孢桿菌屬中。
[Abstract]:32 strains of endophytic bacteria were isolated from the root of Radix Rehmanniae, and 5 strains of Escherichia coli, Staphylococcus aureus, Aspergillus Niger, Rehmannia blight and Rehmannia root rot were selected as the tested strains. The antimicrobial activity was determined by plate confrontation method and bacterial cake method. The results showed that 32 strains of endophytic bacteria isolated from Rehmannia glutinosa had different inhibition ability to 5 kinds of bacteria. Among them, 8 strains of bacteria had inhibitory effect on Escherichia coli, Staphylococcus aureus and Aspergillus Niger, which showed broad-spectrum antimicrobial activity. Two strains of bacteria DH-10 and DH-11 all had inhibitory effects on the five tested bacteria, and had strong antibacterial activity, which had the potential to develop biocontrol bacteria. The isolated active endophytic bacteria were mainly distributed in Bacillus, Pseudomonas and Escherichia coli by 16s rRNA gene sequence analysis. The endophytic bacteria which have antagonistic effect on both macular blight and root rot are mainly concentrated in the genus Bacillus.
【作者單位】： 河南師范大學(xué)生命科學(xué)學(xué)院;資源微生物與功能分子河南省高校重點實驗室培育基地;
【基金】：國家自然科學(xué)基金(編號:21277041、21477035) 河南省高�？萍紕�(chuàng)新團隊支持計劃(編號:13IRTSTHN009)
【分類號】：S476.1

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