本文選題：小花蝽屬 + DNA條形碼技術(shù)��； 參考：《中國生物防治學(xué)報(bào)》2017年04期

【摘要】：本研究利用DNA條形碼技術(shù)對13種小花蝽屬Orius Wolff昆蟲進(jìn)行了鑒定,進(jìn)行了59條COI基因序列堿基組成及種內(nèi)、種間遺傳距離的分析,采用鄰接法、最大簡約法、貝葉斯推論法構(gòu)建了系統(tǒng)發(fā)育樹。結(jié)果表明,小花蝽屬昆蟲COI基因序列堿基組成與典型的昆蟲線粒體DNA一致,A+T平均含量(66.4%)明顯高于G+C含量,密碼子的第3位A+T含量高達(dá)90.7%,堿基替換多為同義替換;13種小花蝽種內(nèi)平均遺傳距離為0.008,種間平均遺傳距離為0.128,種內(nèi)、種間遺傳距離沒有重疊區(qū)域。3種方法構(gòu)建的系統(tǒng)發(fā)育樹的聚類分析與形態(tài)學(xué)鑒定結(jié)果基本一致,除微小花蝽Orius minutus(Linnaeus)可能存在隱存種現(xiàn)象外,其他同一種群的不同個(gè)體單獨(dú)聚為一支。利用DNA條形碼技術(shù)對小花蝽屬昆蟲進(jìn)行物種快速分子鑒定具有可行性。
[Abstract]:In this study, 13 species of Orius Wolff, Orius Wolff, were identified by DNA barcode technique. The composition of 59 COI gene sequences and the genetic distance between species and species were analyzed. A phylogenetic tree was constructed by Bayesian inference. The results showed that the base composition of COI gene sequence was significantly higher than that of the typical insect mitochondrial DNA (66.4%). The third position A T content of codon was as high as 90.7. The average genetic distance was 0.008 in 13 species, 0.128 in species, and 0.128 in intraspecies, and the base substitutions were synonymous, the average genetic distance of 13 species was 0.008, the average genetic distance between species was 0.128, and the average genetic distance between species was 0.128. The cluster analysis of phylogenetic tree constructed by 3 methods of genetic distance between species without overlapping region was consistent with the result of morphological identification, except Orius minutus Linnaeusmay have hidden species. The different individuals of the other same species are clustered into one single branch. It is feasible to use DNA barcode technique to identify the species of Bug.
【作者單位】： 山東省農(nóng)業(yè)科學(xué)院植物保護(hù)研究所/天敵與授粉昆蟲研究中心;
【基金】：國家國際科技合作專項(xiàng)(2013DFA32070) 山東省自然科學(xué)基金(ZR2016CB50) 山東省農(nóng)業(yè)重大應(yīng)用技術(shù)創(chuàng)新專項(xiàng)重點(diǎn)項(xiàng)目 山東省農(nóng)業(yè)科學(xué)院學(xué)科帶頭人科研啟動(dòng)項(xiàng)目
【分類號】：S476.2
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本文編號：2000348