本文選題：雷竹 + 林地覆蓋��； 參考：《林業(yè)科學(xué)》2017年09期

【摘要】：【目的】研究覆蓋對(duì)雷竹林土壤細(xì)菌群落結(jié)構(gòu)及多樣性的影響,為科學(xué)評(píng)價(jià)該種植模式的生態(tài)風(fēng)險(xiǎn)提供科學(xué)依據(jù)�！痉椒ā空{(diào)查并采集不同覆蓋年限(0、3、6、9、12年)雷竹林土樣,采用Illumina Miseq高通量測(cè)序技術(shù)分析16S rDNA土壤細(xì)菌基因V3-V4區(qū)域,鑒定細(xì)菌類群,并結(jié)合測(cè)定土壤的N、P和有機(jī)質(zhì)等化學(xué)指標(biāo)分析覆蓋經(jīng)營(yíng)土壤細(xì)菌群落結(jié)構(gòu)演變及多樣性影響�！窘Y(jié)果】1)測(cè)序共獲得598個(gè)OTUs,計(jì)280 548條讀數(shù),平均讀長(zhǎng)453.5 bp。調(diào)查林地土壤細(xì)菌優(yōu)勢(shì)門為變形菌門、酸桿菌門和放線菌門(平均相對(duì)豐度分別為30.80%、22.0%和13.9%)。2)不同覆蓋年限土樣的多樣性指數(shù)及豐度指數(shù)存在較大差異。覆蓋初期,隨著覆蓋物的投入,林分土壤細(xì)菌的多樣性指數(shù)及豐度指數(shù)均呈增加趨勢(shì),在覆蓋6年時(shí)達(dá)到最大值,之后則呈現(xiàn)一定程度的下降,覆蓋12年與覆蓋初期無較大差別。3)覆蓋對(duì)土壤微生物群落結(jié)構(gòu)造成一定影響,未覆蓋林地土壤細(xì)菌群落結(jié)構(gòu)與覆蓋后林地出現(xiàn)差異;變形菌門豐度由17.60%(對(duì)照)上升為37.77%(覆蓋12年),酸桿菌門豐度呈現(xiàn)下降的趨勢(shì),而放線菌門豐度變化很小。4)覆蓋造成的土壤理化性質(zhì)的改變?cè)谝欢ǔ潭壬蠈?duì)細(xì)菌群落結(jié)構(gòu)可造成影響,隨著覆蓋時(shí)間的增加,細(xì)菌菌落結(jié)構(gòu)與土壤環(huán)境的關(guān)系也趨于復(fù)雜化,覆蓋造成土壤嚴(yán)重酸化,對(duì)照和覆蓋12年的p H值分別為5.23和3.42,但土壤中的嗜酸菌群落酸桿菌門的豐度反而出現(xiàn)下降的趨勢(shì)。5)RDA分析排序軸前兩軸解釋了覆蓋竹林土壤微生物群落變異的88.5%,土壤中各細(xì)菌種群對(duì)土壤環(huán)境因子變化的適應(yīng)性不同,土壤中速效K、全N和有機(jī)質(zhì)的積累會(huì)提高Proteobacteria、Bacteroidetes的相對(duì)豐度,土壤酸化會(huì)降低Chloroflexi的相對(duì)豐度�！窘Y(jié)論】竹林覆蓋經(jīng)營(yíng)對(duì)土壤細(xì)菌群落結(jié)構(gòu)和多樣性產(chǎn)生較大的影響,短期覆蓋(小于6年)能增加土壤細(xì)菌的豐度和多樣性,但隨著覆蓋時(shí)間增加,土壤細(xì)菌的豐度和多樣性下降,土壤各環(huán)境因子的變化會(huì)對(duì)覆蓋竹林土壤微生物群落結(jié)構(gòu)造成影響,對(duì)土壤中微生物多樣性的研究能夠從微生物角度分析土壤退化程度,為竹林土壤修復(fù)提供理論依據(jù)。
[Abstract]:[objective] to study the effect of mulching on the structure and diversity of soil bacterial community in the forest of Phyllostachys praecox, so as to provide a scientific basis for the scientific evaluation of the ecological risk of the planting model. [methods] soil samples of different mulching years were investigated and collected. The V3-V4 region of 16s rDNA soil bacterial gene was analyzed by Illumina Miseq high-throughput sequencing technique. The changes of bacterial community structure and the influence of diversity on soil microbial community were analyzed by using chemical indexes such as NNP and organic matter. [results] 1) A total of 598 OTUs were obtained by sequencing, with 280,548 readings and an average reading length of 453.5 BP. The dominant phylum of bacteria in forest soil was Proteus, and the diversity index and abundance index of soil samples with different mulching years were different between acidobacterium and actinomycetes (average relative abundance was 30.80% and 13.990%, respectively). In the early stage of mulching, the diversity index and abundance index of soil bacteria increased with the mulch input, and reached the maximum value after 6 years of mulching, and then decreased to a certain extent. There was no significant difference between 12 years and the initial period of mulching. 3) the soil microbial community structure was affected to some extent by mulching, but there was a difference between the bacterial community structure of uncovered forest land and the forestland after mulching. The abundance of Proteus increased from 17.60 to 37.77 (covering 12 years). However, the change of soil physical and chemical properties caused by actinomycetes is very small. The relationship between bacterial colony structure and soil environment tends to be complicated with the increase of mulching time, and the change of soil physical and chemical properties can affect the structure of bacterial community to a certain extent, and with the increase of mulching time, the relationship between bacterial colony structure and soil environment is becoming more and more complicated. Soil cover results in severe acidification of the soil, The pH values of control and mulching for 12 years were 5.23 and 3.42, respectively, but the abundance of acidophilus community in soil decreased. The first two axes of RDA analysis explained the variation of soil microbial community in covered bamboo forest. The adaptability of bacterial populations to soil environmental factors was different. The accumulation of available K, total N and organic matter in soil increased the relative abundance of Proteobacteria Bacteroidetes, and soil acidification decreased the relative abundance of Chloroflexi. [conclusion] Bamboo forest cover management has a great effect on the structure and diversity of soil bacterial community. Short-term mulching (less than 6 years) can increase the abundance and diversity of soil bacteria, but with the increase of mulching time, the abundance and diversity of soil bacteria decrease. The change of soil environmental factors will affect the structure of soil microbial community in covered bamboo forest. The study of microbial diversity in soil can analyze the degree of soil degradation from the point of view of microorganism and provide theoretical basis for soil remediation of bamboo forest.
【作者單位】： 國(guó)家林業(yè)局竹子研究開發(fā)中心;
【基金】：林業(yè)公益性行業(yè)科研專項(xiàng)(201504407)
【分類號(hào)】：S714.3

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本文編號(hào)：1998450