本文選題：地衣芽孢桿菌 + 墊料　； 參考：《農(nóng)業(yè)環(huán)境科學(xué)學(xué)報》2017年10期

【摘要】：試驗旨在研究發(fā)酵床飼養(yǎng)模式下,豬日糧中添加地衣芽孢桿菌對發(fā)酵床的理化性質(zhì)和微生物群落的影響。分別飼喂基礎(chǔ)飼糧(對照組)、基礎(chǔ)飼糧+桿菌肽鋅+硫酸黏桿菌素(抗生素組)和基礎(chǔ)日糧+地衣芽孢桿菌(益生菌組)的試驗日糧。結(jié)果表明:飼糧中添加地衣芽孢桿菌能顯著提高發(fā)酵床墊料蛋白酶活性(P0.05),對墊料脲酶活性和銨態(tài)氮含量無顯著影響(P0.05);試驗第15 d時,抗生素組的墊料放線菌數(shù)量顯著低于對照組(P0.05);第35 d時,益生菌組的芽孢桿菌數(shù)量顯著高于其他組(P0.05);第49 d時,益生菌組的芽孢桿菌數(shù)量顯著高于抗生素組(P0.05),抗生素組的葡萄球菌數(shù)量比對照組顯著降低(P0.05);益生菌組、抗生素組的大腸桿菌低于對照組,抗生素組的細菌總數(shù)、芽孢桿菌數(shù)量低于對照組,但差異均不顯著(P0.05),飼糧抗生素一定程度上減少了墊料益生菌,但能有效減少墊料病原菌;各處理組間,墊料微生物DGGE的香農(nóng)-威納指數(shù)、均勻度均無顯著差異(P0.05)。飼用地衣芽孢桿菌顯著增加墊料中芽孢桿菌分布數(shù)量,提高墊料中墊料蛋白酶活,沒有顯著影響墊料細菌多樣性指數(shù),一定程度上提高糞便原位降解效率。
[Abstract]:The purpose of this study was to study the effects of Bacillus licheniformis on the physical and chemical properties and microbial community of fermented bed in pig diet. The experimental diets were fed with basic diet (control group, basic diet of Bacteriopeptide zinc colistin sulfate (antibiotic group) and basal diet of Bacillus licheniformis (probiotics group). The results showed that the addition of Bacillus licheniformis to the diet could significantly increase the protease activity of the fermenting bed pad (P0.05N), but had no significant effect on the urease activity and the ammonium nitrogen content (P0.05N) of the substrate. The number of actinomycetes in the antibiotic group was significantly lower than that in the control group (P 0.05), the number of bacillus in the probiotics group was significantly higher than that in the other groups on the 35th day, and at the 49th day, the number of bacillus in the probiotics group was significantly higher than that in the other groups. The number of Bacillus in probiotics group was significantly higher than that in antibiotic group, and the number of Staphylococcus in antibiotic group was significantly lower than that in control group, while in probiotics group, the number of Escherichia coli in antibiotic group was lower than that in control group, and the total number of bacteria in antibiotic group was lower than that in antibiotic group. The quantity of Bacillus was lower than that of the control group, but the difference was not significant (P 0.05). The diet antibiotics reduced the probiotics in the bedding to some extent, but could effectively reduce the pathogenic bacteria in the cushion. There was no significant difference in evenness (P 0.05). Bacillus licheniformis significantly increased the distribution of Bacillus sp., increased the activity of protease in the gasket, did not significantly affect the bacterial diversity index, and improved the efficiency of in situ degradation of feces to some extent.
【作者單位】： 江蘇省農(nóng)業(yè)科學(xué)院畜牧研究所;
【基金】：江蘇省農(nóng)業(yè)自主創(chuàng)新資金項目[CX13(5040)] 江蘇省農(nóng)業(yè)三新工程項目(SXGC[2016]331)~~
【分類號】：X172

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