微生物固定化載體篩選及其水質處理效果研究
發(fā)布時間:2018-05-16 22:07
本文選題:固定化載體 + 蠟樣芽孢桿菌(Bacillus; 參考:《淡水漁業(yè)》2017年03期
【摘要】:為得到吸附能力強、水質處理效果良好的微生物固定化載體,本實驗沸石、火山石、陶環(huán)、核桃殼、牡蠣殼五種不同載體對蠟樣芽孢桿菌(Bacillus cereus,NY5株)的負載性能,以及在以養(yǎng)殖廢水作為空白對照,游離NY5作為陽性對照的條件下,不同載體固定NY5對養(yǎng)殖的處理效果,并利用主成分分析法對水質處理效果進行綜合評價。結果顯示:沸石和牡蠣殼對NY5的負載性能較好,載菌量分別達1.25×10~9、9.0×10~8CFU/g;在養(yǎng)殖廢水處理方面,火山石NY5組第7天、牡蠣殼NY5組第21天時NH_4~+-N濃度與空白對照相比分別降低53.4%、56.6%;火山石NY5組第7天時NO_3~--N濃度與空白對照相比降低87.9%;第28天時火山石NY5組、牡蠣殼NY5組NO_2~--N濃度與空白對照相比分別降低90.1%、80.2%;第14天時火山石NY5組TN濃度與空白對照相比降低51.6%。從主成分分析的結果也可以看出,火山石NY5組和牡蠣殼NY5組對養(yǎng)殖廢水綜合處理效果較好。
[Abstract]:In order to obtain the immobilized microorganism carrier with strong adsorption ability and good water quality treatment effect, the loading performance of zeolite, volcanic stone, pottery ring, walnut shell and oyster shell on Bacillus cereusnyne NY5 strain was studied. Under the condition that culture wastewater was used as blank control and free NY5 was used as positive control, different carriers fixed NY5 were used to treat culture, and principal component analysis was used to evaluate the effect of water quality treatment. The results showed that the loading capacity of zeolite and oyster shell on NY5 was better, and the amount of bacteria carrying reached 1.25 脳 10 ~ (10) ~ (99) 脳 10 ~ (8) CFU / g, respectively. In the treatment of aquiculture wastewater, the volcanic stone NY5 group was treated on the 7th day. The NH4- N concentration of oyster shell NY5 group was 53.4% lower than that of the blank control group on the 21st day, the NO_3~--N concentration of the volcanic stone NY5 group was 87.9% lower than that of the blank control group on the 7th day, and the NY5 group on the 28th day. The concentration of NO_2~--N in the NY5 group of oyster shell decreased 90.1% 80.2 compared with the control group, and the TN concentration in the NY5 group decreased 51.6% on the 14th day compared with the control group. From the results of principal component analysis, it can be seen that the NY5 group and the NY5 group of oyster shell are better in the comprehensive treatment of culture wastewater.
【作者單位】: 大連海洋大學水產與生命學院;中國水產科學研究院珠江水產研究所農業(yè)部熱帶亞熱帶水產資源利用與養(yǎng)殖重點實驗室;
【基金】:現(xiàn)代農業(yè)產業(yè)技術體系專項資金(CARS-49) 廣東省海洋漁業(yè)科技與產業(yè)發(fā)展專項科技攻關與研發(fā)項目(A201401B04)
【分類號】:X172;X714
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