本文選題：谷子 + 干旱��； 參考：《遼寧大學(xué)》2017年碩士論文

【摘要】：干旱(Drought)是全世界最普遍的自然現(xiàn)象,并且是對(duì)農(nóng)作物傷害最嚴(yán)重的脅迫之一。近年來(lái),人們對(duì)干旱引起的作物減產(chǎn)問(wèn)題越來(lái)越重視,抗旱研究已成為全球的研究熱點(diǎn)。植物對(duì)干旱的響應(yīng)體現(xiàn)在生長(zhǎng)發(fā)育的各個(gè)階段。許多研究結(jié)果發(fā)現(xiàn)表觀遺傳修飾可以影響植物的生長(zhǎng)發(fā)育。尤其是DNA甲基化作為表觀遺傳修飾的重要組成成員,對(duì)于基因表達(dá)的調(diào)控,以及基因組防御等過(guò)程有重要作用。已有研究發(fā)現(xiàn)逆境脅迫可以引起植物DNA甲基化水平以及模式的變異。為了對(duì)植物響應(yīng)干旱脅迫的表觀遺傳效應(yīng)進(jìn)一步研究,并為了對(duì)干旱脅迫表觀遺傳機(jī)制的了解更加全面,發(fā)現(xiàn)植物可能的抗旱功能基因,本研究以抗旱種質(zhì)谷子品種豫谷1為實(shí)驗(yàn)材料,利用20%PEG模擬干旱脅迫條件,對(duì)谷子脅迫處理2h、6h、12h、24h、3d、5d后,隨機(jī)混取各個(gè)時(shí)間點(diǎn)的葉片DNA,運(yùn)用MSAP技術(shù)進(jìn)行DNA甲基化水平和模式檢測(cè),并且對(duì)干旱脅迫引起的變異進(jìn)行分析。本研究共獲得1520個(gè)清晰的位點(diǎn),擴(kuò)增條帶1476-1502條,甲基化水平的變化范圍為10.92%-11.05%,其中半甲基化位點(diǎn)30-57個(gè),占總位點(diǎn)的1.97%-3.75%,全甲基化位點(diǎn)110-138個(gè),占總位點(diǎn)的7.24%-9.08%,表明甲基化在谷子中甲基化模式更多的是全甲基化。根據(jù)甲基化位點(diǎn)分析發(fā)現(xiàn),CG位點(diǎn)的甲基化水平比CNG位點(diǎn)以及CG/CNG位點(diǎn)分別高出3.9%和3.7%,說(shuō)明甲基化多數(shù)發(fā)生在CG位點(diǎn)上。干旱脅迫引起的甲基化變異位點(diǎn)占總位點(diǎn)的0.52%-2.11%,其中發(fā)生超甲基化變化的位點(diǎn)占總位點(diǎn)的0.13%-0.54%,而發(fā)生去甲基化的位點(diǎn)占總位點(diǎn)的0.13%-1.78%。根據(jù)發(fā)生甲基化變異的位點(diǎn)分析發(fā)現(xiàn),甲基化變異主要發(fā)生在CNG位點(diǎn),CNG位點(diǎn)發(fā)生超甲基化的概率為0.07%-0.20%,而發(fā)生去甲基化的概率為0.13%-1.64%。對(duì)甲基化變異帶進(jìn)行回收測(cè)序,Blast N和Blast X分析結(jié)果表明,干旱脅迫引起的谷子甲基化變異序列與已知谷子中過(guò)氧化酶基因同源,表明谷子在應(yīng)對(duì)干旱脅迫時(shí),甲基化變異與植物體內(nèi)相關(guān)蛋白基因相互作用從而適應(yīng)脅迫。
[Abstract]:Drought Droughtis the most common natural phenomenon in the world and is one of the most serious stresses on crops. In recent years, people pay more and more attention to crop yield reduction caused by drought, and drought resistance research has become a global research hotspot. The response of plants to drought occurs at all stages of growth and development. Many studies have found that epigenetic modification can affect plant growth and development. Especially, DNA methylation is an important component of epigenetic modification, which plays an important role in the regulation of gene expression and genome defense. It has been found that stress stress can induce the variation of DNA methylation level and pattern in plants. In order to further study the epigenetic effect of plant response to drought stress and to understand the epigenetic mechanism of drought stress, the possible drought resistance functional genes were found. In this study, the drought-resistant germplasm variety Yugu 1 was used as the experimental material, and 20%PEG was used to simulate the drought stress condition. The leaf DNA of each time point was randomly mixed for 5 days after 2 h ~ 6 h ~ (12) h ~ (24) d ~ (-1) stress, and the DNA methylation level and model were detected by MSAP technique. The variation caused by drought stress was analyzed. In this study, 1 520 clear sites were obtained and 1476-1502 bands were amplified. The range of methylation level was 10.92-11.05, of which 30-57 were semi-methylation sites, which accounted for 1.97-3.75 and 110-138 completely methylated sites. 7.24- 9.08% of the total sites indicated that the methylation pattern in millet was more total methylation. According to the analysis of methylation site, the methylation level of CG site was 3.9% and 3.7% higher than that of CNG site and CG/CNG site, respectively, indicating that most of the methylation occurred at CG site. The methylation variation sites caused by drought stress accounted for 0.52-2.11 of the total sites, among which the hypermethylation sites accounted for 0.13-0.54 of the total sites, while the demethylation sites accounted for 0.13-1.78 sites of the total sites. According to the site analysis of methylation mutation, the probability of hypermethylation occurred mainly at CNG locus was 0.07-0.20, and the probability of demethylation was 0.13- 1.64g. The methylation mutation bands were recovered and sequenced by blast N and Blast X analysis. The results showed that the methylation variation sequence of millet caused by drought stress was homologous to the known peroxidase gene in millet, which indicated that millet was in response to drought stress. Methylation variation interacts with plant associated protein genes to adapt to stress.
【學(xué)位授予單位】：遼寧大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S515;S423

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 周廣勝;;氣候變化對(duì)中國(guó)農(nóng)業(yè)生產(chǎn)影響研究展望[J];氣象與環(huán)境科學(xué);2015年01期

2 王尊欣;張樹(shù)珍;;作物抗旱性及抗旱育種研究進(jìn)展[J];作物雜志;2014年02期

3 張雁明;劉曉東;馬建萍;溫琪汾;韓淵懷;;谷子抗旱研究進(jìn)展[J];山西農(nóng)業(yè)科學(xué);2013年03期

4 劉桂紅;王玨;杜金哲;管延安;;谷子萌芽期抗旱性鑒定研究[J];中國(guó)農(nóng)學(xué)通報(bào);2013年03期

5 趙云雷;葉武威;王俊娟;樊保香;宋麗艷;;DNA甲基化與植物抗逆性研究進(jìn)展[J];西北植物學(xué)報(bào);2009年07期

6 唐益苗;趙昌平;高世慶;田立平;單福華;吳敬新;;植物抗旱相關(guān)基因研究進(jìn)展[J];麥類作物學(xué)報(bào);2009年01期

7 彭方仁;楊玉珍;朱振賢;;干旱脅迫對(duì)不同種源香椿葉片膜脂過(guò)氧化和保護(hù)酶系統(tǒng)的影響[J];植物資源與環(huán)境學(xué)報(bào);2007年02期

8 劉華玲;馬欣榮;;植物抗旱分子機(jī)理研究進(jìn)展[J];世界科技研究與發(fā)展;2006年06期

9 康宗利;楊玉紅;張立軍;;植物響應(yīng)干旱脅迫的分子機(jī)制[J];玉米科學(xué);2006年02期

10 丁一匯;任國(guó)玉;石廣玉;宮鵬;鄭循華;翟盤(pán)茂;張德二;趙宗慈;王紹武;王會(huì)軍;羅勇;陳德亮;高學(xué)杰;戴曉蘇;;氣候變化國(guó)家評(píng)估報(bào)告(Ⅰ):中國(guó)氣候變化的歷史和未來(lái)趨勢(shì)[J];氣候變化研究進(jìn)展;2006年01期

相關(guān)碩士學(xué)位論文 前1條

1 劉煒;干旱脅迫誘導(dǎo)的水稻基因組胞嘧啶甲基化變化[D];東北師范大學(xué);2006年

，

本文編號(hào)：1841687